Figure 1From: Estimates of sensitivity and specificity can be biased when reporting the results of the second test in a screening trial conducted in series Flowchart for single test design. Flowchart depicts a single test screening trial from an omniscient point of view. Dashed lines indicate a pathway that is unavailable to that class of participants (true case or true non-case) due to the assumptions of our model. The gray box indicates cases that are misclassified as noncases by the study investigator.Back to article page