Table 4 Saliva and saliva DNA yield, purity and genotyping rate by sample clarity, COMPASS participants
From: Clinical trial participant characteristics and saliva and DNA metrics
Saliva | DNA | |||||
|---|---|---|---|---|---|---|
Sample variable | Clear | Cloudy | P* | Clear | Cloudy | P* |
N samples | 375 | 158 | 446 | 93 | ||
Saliva volume mean (SD), ml | ||||||
4.5 (1.1) | 4.7 (1.1) | 0.037 | 4.4 (1.0) | 5.2 (1.0) | <0.001 | |
DNA based measurements mean (SD) | ||||||
UV, mean (SD), ug | 121.7 (111.5) | 236.4 (210.5) | <0.001 | 120.2 (104.2) | 323.0 (235.8) | <0.001 |
PG, mean (SD), ug | 69.3 (62.9) | 115.6 (102.6) | <0.001 | 64.2 (55.5) | 171.8 (111.0) | <0.001 |
qPCR, mean (SD), ug | 54.1 (51.2) | 79.4 (70.1) | <0.001 | 49.1 (44.5) | 121.4 (78.3) | <0.001 |
A260/280 | 1.84 (0.10) | 1.84 (0.12) | 0.746 | 1.84 (0.11) | 1.83 (0.11) | 0.617 |
% human DNA | 78.0 (16.4) | 71.3 (17.6) | <0.001 | 77.0 (16.9) | 72.0 (17.4) | 0.010 |
Genotype (%) | 98.5 (9.6) | 98.7 (7.4) | 0.757 | 98.4 (9.7) | 99.4 (4.0) | 0.104 |