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Table 4 Quantity and quality measurements of nucleic acids

From: Quantity and quality of nucleic acids extracted from archival formalin fixed paraffin embedded prostate biopsies

Archival biopsies

RNA

DNA

FFPE tissue input

10 μm

10 μm

Number of extractions

84

84

Yield (ng/μl)

 Minimum

1.6

0.6

 Maximum

30.9

12.5

 Median

9.2

3.3

 IQR

6.35

2.3

A260/A280

 Minimum

0.7

1.1

 Maximum

1.9

5.3

 Median

1.5

1.7

 IQR

0.2

0.4

RIN-value

 Minimum

1.0

–

 Maximum

4.1

–

 Median

2.5

–

 IQR

0.18

 

CT -value RNA18S5

 Minimum

22.0

24.6

 Maximum

39.3

38.7

 Mean

28.8

–

 SD

3.1

–

 Median

–

29.2

 IQR

–

6.4

  1. Quantity and quality measurements of nucleic acids isolated from archival FFPE biopsy tissues from the prostate tumor. SD Standard Deviation, IQR Inter Quartile Range
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BMC Medical Research Methodology

ISSN: 1471-2288

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