 Research Article
 Open Access
 Published:
Assessment of predictive performance in incomplete data by combining internal validation and multiple imputation
BMC Medical Research Methodology volume 16, Article number: 144 (2016)
Abstract
Background
Missing values are a frequent issue in human studies. In many situations, multiple imputation (MI) is an appropriate missing data handling strategy, whereby missing values are imputed multiple times, the analysis is performed in every imputed data set, and the obtained estimates are pooled. If the aim is to estimate (added) predictive performance measures, such as (change in) the area under the receiveroperating characteristic curve (AUC), internal validation strategies become desirable in order to correct for optimism. It is not fully understood how internal validation should be combined with multiple imputation.
Methods
In a comprehensive simulation study and in a real data set based on blood markers as predictors for mortality, we compare three combination strategies: ValMI, internal validation followed by MI on the training and test parts separately, MIVal, MI on the full data set followed by internal validation, and MI(y)Val, MI on the full data set omitting the outcome followed by internal validation. Different validation strategies, including bootstrap und crossvalidation, different (added) performance measures, and various data characteristics are considered, and the strategies are evaluated with regard to bias and mean squared error of the obtained performance estimates. In addition, we elaborate on the number of resamples and imputations to be used, and adopt a strategy for confidence interval construction to incomplete data.
Results
Internal validation is essential in order to avoid optimism, with the bootstrap 0.632+ estimate representing a reliable method to correct for optimism. While estimates obtained by MIVal are optimistically biased, those obtained by MI(y)Val tend to be pessimistic in the presence of a true underlying effect. ValMI provides largely unbiased estimates, with a slight pessimistic bias with increasing true effect size, number of covariates and decreasing sample size. In ValMI, accuracy of the estimate is more strongly improved by increasing the number of bootstrap draws rather than the number of imputations. With a simple integrated approach, valid confidence intervals for performance estimates can be obtained.
Conclusions
When prognostic models are developed on incomplete data, ValMI represents a valid strategy to obtain estimates of predictive performance measures.
Background
The aim of a prognostic study is to develop a classification model from an available data set and to estimate the performance it would have in future independent data, i.e., its predictive performance. This cannot be achieved by fitting the model on the whole data set and evaluating performance in the same data set, since a model generally performs better for the data used to fit the model than for new data (“overfitting”) and performance would thus be overestimated. This can be observed already in lowdimensional situations and is especially pronounced in relatively small data sets [1, 2]. Instead, the available data have to be split in order to allow performance assessment in a part of the data that has not been involved in model fitting [3, 4]. For efficient sample usage, this is often achieved by internal validation strategies such as bootstrapping (BS), subsampling (SS) or crossvalidation (CV).
The task of assessing predictive performance is made even more complicated when the data set is incomplete. Missing values occur frequently in epidemiological and clinical studies, for reasons such as incomplete questionnaire response, lack of biological samples, or resourcebased selection of samples for expensive laboratory measurements. The majority of statistical methods, including logistic regression models, assume a complete data matrix, so that some action is required prior to or during data analysis to allow usage of incomplete data. Since ad hoc strategies such as completecase analysis and single imputation often provide inefficient or invalid results, and modelbased strategies require often sophisticated problemspecific implementation, multiple imputation (MI) is becoming increasingly popular among researchers of different fields [5, 6]. It is a flexible strategy that typically assumes missing at random (MAR) missingness, that is, missingness depending on observed but not unobserved data, which is often, at least approximately, given in practice [5]. MI involves three steps [7]: (i) missing values are imputed multiple (M) times, i.e., missing values are replaced by plausible values, for instance derived as predicted values from a sequence of regression models including other variables, (ii) statistical analysis is performed on each of the resulting completed data sets, and (iii) the M obtained parameter estimates and their variances are pooled, taking into account the uncertainty about the imputed values [8].
When the estimate of interest is a measure of predictive performance of a classification model, or a measure of incremental predictive performance of an extended model as compared to a baseline model, the application of MI is not straightforward. Specifically, it is unclear how internal validation and MI should be combined in order to obtain unbiased estimates of predictive performance.
Previous strategies combining internal validation with MI mostly focused on application without the aim to compare their chosen strategy against others or to assess their validity [9–11]. Musoro et al. [12] studied the combination of BS and MI in the situation of a nearly continuous outcome using LASSO regression, essentially reporting that the strategy of conducting MI first followed by BS on the imputed data yielded overoptimistic mean squared errors, whereas conducting BS first on the incomplete data followed by MI yielded slightly pessimistic results in the studied settings. Wood et al. [13] presented a number of strategies for performance assessment in multiply imputed data, leaving, however, the necessity of validating the model in independent data to future studies. Hornung et al. [14] examined the consequence of conducting a single imputation on the whole data set as compared to the training data set on crossvalidated performance of classification methods, observing a negligible influence. Their investigation was restricted to one type of imputation that did not include the outcome in the imputation process.
In this paper, we present results of a comprehensive simulation study and results of a real databased simulation study comparing various strategies of combining internal validation with MI, with and without including the outcome in the imputation models. Our study extends upon previous work with regard to several aspects: (1) We consider different internal validation strategies and different ways to correct for optimism, we (2) study measures of discrimination, calibration and overall performance as well as incremental performance of an extended model, and we (3) closely examine the sensitivity of the results towards characteristics of the data set, including sample size, number of covariates, true effect size and degree and mechanism of missingness. Furthermore, we (4) elaborate on the number of imputations and resamples to be used and (5) provide an approach for the construction of confidence intervals for predictive performance estimates. Finally, we (6) translate our results into recommendations for practice, considering the applicability of the proposed methods for epidemiologists with limited analytical and computational resources.
Methods
Study data
Two simulation studies were conducted: In the first, incomplete data were generated de novo with different (known) effect sizes, facilitating the comparison of predictive performance estimates of different combined validation/imputation strategies against the respective true performance measure. The second simulation study was based on the complete observations of a real incomplete data set, in which we introduced missing values in a pattern mirroring that of the whole incomplete data set, aiming to compare strategies in a realistic data situation.
Simulation study 1: de novo simulation
Data generation
Data were generated according to a variety of settings, covering a large spectrum of practically occurring data characteristics (Table 1). For each setting, 250 data sets were randomly generated. Two situations were investigated. In situation 1, only one set of covariates was considered (the number of which is denoted as p), with the aim being the estimation of predictive performance of a model comprising this set of covariates. In situation 2, two sets of covariates were considered (with p_{0} the number of baseline covariates and p_{1} the number of additional covariates), in order to study the estimation of added predictive performance of the model comprising both sets of covariates as compared to a model containing only the p_{0} baseline covariates.
For each simulated data set, a binary outcome vector y=(y_{1},y_{2},…,y_{ n }) was created with the prespecified case probability frac. A covariate matrix X=(x_{1},x_{2},…,x_{ n }) was simulated by drawing n times from a p or p_{0}+p_{1}dimensional (in situations 1 and 2, respectively) multivariate normal distribution with mean vector 0 and variancecovariance matrix Σ with variances equal to 1 and covariances specified by the correlation among variables (ρ in situation 1, ρ_{0} and ρ_{1} for the baseline and additional covariates, respectively, in situation 2) as provided in Table 1. Then, effect sizes were introduced in a way that each set of covariates achieved an (added) performance approximately in the magnitude of a prespecified area under the receiveroperating characteristic (ROC) curve (AUC) value. As a reference we used the theoretical relationship [15]:
where Δμ denotes the vector of mean differences in covariate values to be introduced between both outcome classes, i.e., Δμ=E(x_{ i }y_{ i }=1)−E(x_{ i }y_{ i }=0), and Φ the standard normal cumulative distribution function. We used a simplified scenario with a unique effect size chosen for all covariates within each set, i.e., Δμ=(Δμ,Δμ,…,Δμ) in situation 1, and Δμ=(Δμ_{0},Δμ_{0},…,Δμ_{0},…,Δμ_{1},Δμ_{1},…,Δμ_{1}) in situation 2, and found Δμ by solving Eq. (1) numerically using the R function uniroot. Then, we added Δμ/2 to the cases’ covariate values, and substracted Δμ/2 from the controls’ covariate values, in order to achieve an average difference of Δμ in covariate values between cases and controls. Using this procedure, we implicitly model the outcome y_{ i } as follows: P(y_{ i }=1x_{ i })=logistic(γ·x_{ i }), where x_{ i } denotes the vector of covariate values for observation i, i=1,…,n, γ=Σ^{−1}·Δμ a pdimensional (situation 1) or p_{0}+p_{1}dimensional (situation 2) vector of coefficients, and \(\text {logistic}(x) = \frac {e^{x}}{1+e^{x}}\) the logistic function.
Imposing missingness
Different degrees of missingness (see Table 1) were introduced separately to the sets of covariates (one set in situation 1 with proportion of missing values denoted as miss; two sets in situation 2 with proportion of missing values in the baseline and additional covariates denoted as miss_{0} and miss_{1}, respectively; to improve readability, we use the parameter notations of situation 1 below) according to three different mechanisms frequently occurring in practice: missing completely at random (MCAR), where missingness occurs independently of any observed or missing values, missing at random (MAR), where missingness of variables depends on observed values including outcome values but not on the unknown values of the missing data, and blockwise missing at random (MARblock), where blocks of variables share their missingness pattern. We did not consider missingness in the outcome.
MCAR missingness was created by randomly introducing the prespecified proportion miss of missing values into the covariates. To achieve MAR missingness, we used an approach similar to that applied by Marshall et al. [16]. Let X_{ ij } denote the j^{th} covariate for observation i, with i=1,…,n and j=1,…,p, and M_{ ij } the indicator for its missingness. Then, the probability of missingness for each covariate value was modeled as a function of the value of one other covariate, of missingness of another covariate, and of the outcome value.
where \(X_{ik_{j}}\) denotes the observation of a randomly chosen other covariate and y_{ i } the binary outcome value. Without loss of generality, missingness of the previous (j−1th) covariate was used for technical reasons (missingness available). β_{1j} was defined as
and β_{2j} as
The intercepts β_{0j} were estimated by numerically solving the equation
for each j. To achieve the proportion of missing values miss exactly, values were set to missing by drawing n×miss times from a multinomial distribution with probability vector (P(M_{ ij }=1))_{i=1,…,n}.
Finally, we created a missingness structure similar to that observed in our application data, that is, a block structure of missingness (MARblock). In practice, such a structure can occur when groups of laboratory parameters are measured for certain groups of subjects defined by other variables (see below). Approximate blockwise missingness was simulated with missingness of variables assigned to each block depending on covariates outside the block. Variables were randomly assigned to three blocks, and probability of missingness modified as follows for the covariates j in each block b, b=1,…,3:
where for each covariate j within block b the same covariate \(X_{k_{b}}\) was chosen among covariates outside the block, leading to similarly high/low probabilities for all covariates in the respective block. The exact proportion of missing values miss was again achieved by drawing from a multinomial distribution, as described for MAR above. Example R code for simulation study 1 is available in Additional file 2.
Simulation study 2: real databased simulation
Data set
Data were obtained from the populationbased research platform MONICA (MONItoring of trends and determinants in CArdiovascular disease)/ KORA (COoperative health research in the Region of Augsburg), surveys S1 (1984/85), S2 (1989/90) and S3 (1994/95), comprising individuals of German nationality aged 25 to 74 years. The study design and data collection have been described in detail elsewhere [17]. Written informed consent was obtained from all participants and the studies were approved by the local ethics committee.
In a random subcohort comprising 2225 participants aged 35 to 74 years, blood concentrations of 15 inflammatory markers were measured [18–20] as part of a casecohort study assessing potential risk factors for cardiovascular diseases and type 2 diabetes. In the present analysis, allcause mortality was used as the outcome. To achieve a largely healthy population at baseline, subjects with a history of stroke, myocardial infarction, cancer or diabetes at baseline were excluded. Among the remaining 2012 subjects, 294 (14.6 %) died during the 15year followup period. Average survival time among the deceased participants was 9.0 years (range 0.2 to 15.0 years), and three participants were censored at 2.7, 6.9 and 7.9 years. See Additional file 1: Table S1 for a description of baseline phenotypes including the inflammatory markers.
Whereas all other variables were almost completely observed (less than 0.4 % missing entries for each variable), missingness among the 15 inflammationrelated markers was 7.2 % on average (range 0.2 – 26.4 %, see Additional file 1: Table S1), 37.2 % of observations had missing entries in inflammationrelated markers, with missingness ranging from 0 to 93.3 %. The missingness pattern showed a block structure (Fig. 1), owing to the fact that measurement of inflammatory markers was conducted in different laboratory runs – for which samples were selected based on sample availability at the time of measurement. Five blocks of covariates could be roughly distinguished: Block 1, comprising CRP, without missings, block 2, conprising ICAM, ESelectin, IL6, MCP1, IL18,IP10 and IL8, block 3, comprising RANTES and MIF, block 4, comprising leptin, MPO, TGF β1 and Adiponectin, and block 5, comprising 25(OH)D. Similarly, observations could be assigned to five patterns of missingness: pattern 1, comprising observations with a missing entry only for block 2, 3, 4 and 5 variables, pattern 2, only for block 4 and 5 variables, pattern 3, only for block 4 variables, pattern 4, only for block 3 and 5 variables, and pattern 5, only for the block 5 variable 25(OH)D.
Imposing missingness
To use the MONICA/KORA subcohort as the basis for the real databased simulation study, we first investigated determinants of missingness in inflammationrelated markers in the full subcohort, followed by imposing missingness on the data set consisting of the complete observations only (n=1258) in a way that yielded a missingness pattern closely resembling the block structure and the relations in the original data set. In detail, we used the five patterns of missingness described above as a basis, and, for each pattern, identified other variables in the data set correlated (Kendall’s τ) with the respective pattern indicator (1 for observations that are part of the respective pattern; 0 else). Consequently, we selected those variables showing an absolute correlation above 0.1: sex and survey 1 for pattern 1, survey 1 for pattern 2, sex, survey 1 and alcohol intake for pattern 3, and no covariates for pattern 4 and 5. 250 simulations were conducted. In each simulation, a proportion of complete observations was assigned to each pattern identical to the proportion observed in the original data set. This was achieved by modeling pattern indicators as a function of the respective correlated variable(s) in the full incomplete data set in a logistic regression model, and predicting pattern membership probability of the respective pattern for the observations in the completeobservation data set. To achieve the aspired proportion of observations newly assigned to each pattern exactly, we drew the required number of times from a multinomial distribution with the predicted probability vector. Finally, for observations assigned to pattern 1, all variables of blocks 2, 3, 4 and 5 were set to missing, for pattern 2, variables of blocks 4 and 5, and so on, according to the definitions above. The resulting data sets showed a missingness pattern closely resembling that of the original data set (shown for the first 12 simulation runs in Additional file 1: Figure S1).
Imputation
We used the multiple imputation by chained equations (MICE) framework [7, 21]. It is based on the principle of a repeated chain of regression equations through the incomplete variables, where in each imputation model, the respective incomplete variable is modeled as a function of the remaining variables. Arbitrary regression models can be used. We applied predictive mean matching for all incomplete (continuous) variables. It is based on Bayesian linear regression, where after modeling, the posterior predictive distribution of the data is specified and used to draw predicted values [22]. Then, missing values are replaced by a random draw of observed values of that variable from other observations with the closest predicted values (default: the five closest values). In each imputation model, all other variables (and, in the databased simulation study, quadratic terms of continuous variables, passively imputed themselves) were included as covariates. Before imputation, to improve normality of the continuous incomplete variables, distributions of raw, natural logarithm, cubic root and square root transformed variables were tested for normality using ShapiroWilk tests, and the transformation yielding the maximum test statistic was applied.
Depending on the strategy used (see below), the outcome was included (strategy MI) or not included (strategy MI(y)) in the imputation models. If MI was not combined with internal validation, a pooled performance estimate was obtained by averaging the performance estimates \(\hat \theta ^{(m)}\), m=1,…,M, from the M imputed data sets, according to Rubin [8]. Example R code for the conduction of MICE is available in Additional file 2.
Internal validation strategies
Three internal validation strategies were considered: bootstrapping (BS), subsampling (SS) and Kfold crossvalidation (CV). The principles underlying the three strategies are visualized for complete data in Fig. 2a (BS) and in Additional file 1: Figure S2 (SS, CV).
Briefly, in BS, B bootstrap samples are drawn with replacement from the original sample, so that each BS sample will contain certain observations more than once, and others not at all. The average proportion of independent observations included in each BS sample is asymptotically 63.2 % [23]. The approx. 36.8 % remaining observations are frequently referred to as the outofbag (OOB) sample. To get an estimate for predictive performance from BS, several strategies were proposed (Fig. 2). First, the optimism of the apparent performance \(\hat \theta ^{\text {Orig,Orig}}\) (i.e., the performance of the model in the original data after using the whole original data set for model fitting), can be estimated as difference between average apparent performance in the BS samples and average performance of models fitted in each BS sample evaluated in the original sample [3]: \(\widehat {{optimism}}= \hat \theta ^{{BS,BS}}  \hat \theta ^{{BS,Orig}}\). Accordingly, an “optimismcorrected” (opt.corr.) measure for predictive performance, sometimes referred to as ordinary bootstrap estimate, can be obtained by subtracting the estimated optimism from apparent performance in the original data: \(\hat \theta ^{{opt.corr.}} = \hat \theta ^{{Orig,Orig}} \widehat {{optimism}}\). Second, the model can be fitted on the BS samples and evaluated on the OOB samples (\(\hat \theta ^{\text {OOB}}\)). The resulting performance estimate tends to underestimate performance since less information was used in the model fitting step than provided in the full data [24]. Thus, the BS 0.632+ estimate (\(\hat \theta ^{\text {0.632+}}\)) has been proposed as a weighted average of apparent and OOB performance:
with weights \(w = \frac {0.632}{10.368 \cdot R}\) depending on the relative overfitting rate \(R=\frac {\hat \theta ^{{OOB}}\hat \theta ^{{Orig,Orig}}}{\hat \theta ^{{noinfo}}\hat \theta ^{{Orig,Orig}}}\) (Fig. 2, [23]). This requires that we know the performance of the model in the absence of an effect (\(\hat \theta ^{\text {noinfo}}\)), which is either known (e.g., 0.5 in the case of the AUC, and 0 in the case of added predictive performance measures) or can be approximated as the average performance measure with randomly permuted outcome prediction. We used 1000 permutations to assess \(\hat \theta ^{\text {noinfo}}\) for the Brier score. In addition, we considered the BS 0.632 estimate \(\hat \theta ^{\text {0.632}} = 0.368 \cdot \hat \theta ^{{Orig,Orig}} + 0.632 \cdot \hat \theta ^{\text {OOB}}\) [25].
SS and CV involve drawing without replacement. For SS, we sampled a proportion 63.2 % of samples for model fitting, leaving again 36.8 % for evaluation. The optimism correction methods described for the BS can be directly translated to SS. For Kfold CV, the sample is split in K equally sized parts, and for each of the parts, the remaining K−1 parts are used for model fitting and the leftout part for evaluation of the model, followed by averaging the performance estimates obtained from the K runs. We used K=3 and K=10, with the former being comparable to BS in terms of the proportion of independent observations in the training sets, and the latter being a popular choice in the literature. Repeating Kfold CV B times and averaging the resulting performance estimates might improve stability of performance evaluation [2]. Thus, both simple (CV3, CV10) and repeated (CV3rep, CV10rep) CV with K=3 and K=10, respectively, were included in the investigation.
Combination of internal validation with multiple imputation
Simulated and real incomplete data were analyzed according to three combination strategies: Internal validation data splits followed by MI of the training/fitting and testing/evaluation data parts separately (ValMI), and performing the internal validation on multiply imputed data with (MIVal) and without (MI(y)Val) having included the outcome in the imputation models. Thereby Val represents the different validation strategies used, i.e., BS, SS, CVK and CVKrep. A visualization is provided for BS in Fig. 2. When performing MI, it is generally recommended to use the outcome data y in the imputation models for missing covariates (i.e., method MIVal) [26]. However, in the present context, where we split the imputed data into a training and an evaluation set (Val), we may want to consider removing y from the imputation models (i.e., method MI(y)Val) because these models are fit to the whole data set, including the data that will become part of the evaluation set (i.e., the OOB or testing set). Dropping y from the imputation models keeps the evaluation set blind to the outcomecovariate relationship in the training set. This is by default the case for ValMI, where training and testing parts of the data set are imputed separately, so we did not consider ValMI(y).
For comparison, we also analyzed data using simple MI and MI(y) without internal validation. In addition, strategies were compared to internal validation (Val) in complete data, where possible. Since we did not observe changes in variability across the simulations when values were increased beyond B=10 and M=5,B=10 validation samples and M=5 imputations were used for BS and SS for incomplete data, and B=50 for complete data in the simulation studies. For CV, none (B=1) or B=5 repetitions and M=5 were used for incomplete, and B=1 or 25 repetitions for complete data. Note that these do not represent choices for B and M in practice, but that lower numbers can be used for simulation where variability across the 250 simulated data sets exceeds resampling and imputation variability within each data set.
Modeling and performance measures
There is no unique definition for the performance of a prediction model. Three types of performance measures can be distinguished: measures of model discrimination, the ability of a model to separate outcome classes, i.e., to assign cases a higher risk than controls, measures of calibration, the unbiasedness of outcome predictions, in a way that of the observations with a predicted outcome probability of pr, about a fraction pr are cases, and measures of overall performance, the distance between observed and predicted outcome [3, 4].
We considered selected measures of each type for the binary (logistic) prediction model in the de novo simulation study. Of note, the focus was not on assessing the appropriateness of the different performance criteria in general, but rather to evaluate their estimation in the presence of missing values as compared to complete data.
As a discrimination measure, we considered the area under the ROC curve (AUC), which determines the probability that the model assigns a randomly chosen case (or, in more general terms, observation with outcome y=1) a higher predicted outcome probability than a randomly chosen control (observation with outcome y=0) and is equal to the concordance (c) statistic in the case of a binary outcome [4, 27]. As calibration measures, we used intercept and slope of a logistic regression model of observed against predicted outcomes, with deviation from 0 and 1, respectively, indicating suboptimal calibration [11, 28]. Finally, as overall performance measures we considered the Brier score, i.e., the average squared difference between observed and predicted outcomes, \(\text {Brier} = \frac {1}{n}\sum _{i=1}^{n}{\left (y_{i}  \hat y_{i}\right)^{2}}\) [4, 29].
To assess added predictive performance of an extended as compared to a baseline model, we considered change in discrimination (ΔAUC) and three measures based on risk categories. These included, first, the net reclassification improvement (NRI), i.e., the difference between the proportion of observations moving into a ‘more correct’ risk category (i.e., cases moving up, controls moving down) and the proportion of observations moving into a ‘less correct’ risk category with the extended as compared to the baseline model [30]. This requires the definition of risk categories, where a single cutoff below the disease risk in the study population renders NRI by trend a measure for improvement in the classification of controls, and a single cutoff above the disease risk makes it a measure for improvement in the classification of cases [31]. In order to capture both, we chose three categories, [0, \(\frac {1}{2}{frac}\)], [\(\frac {1}{2}{frac}, \frac {3}{2}{frac}\)], [\(\frac {3}{2}{frac}\),1], where frac (≤0.5, without loss of generality, since the NRI is not sensitive towards class label assignment) denotes the outcome case frequency in the data set (see Table 1 for simulation study). Second, we used the continuous NRI, a categoryfree version of the NRI [32], and lastly, the integrated discrimination improvement (IDI), which equals the integrated NRI over all possible risk cutoffs [30].
In the datadriven simulation study, the ability of inflammationrelated markers to predict allcause mortality was assessed using a Cox proportional hazards model, with and without additional inclusion of covariates known to be relevant for mortality prediction (age, sex, survey, BMI, systolic blood pressure, total to high density lipoprotein (HDL) cholesterol ratio, smoking status, alcohol intake and physical activity). To acknowledge potential nonlinear effects, quadratic terms were additionally included for all continuous variables. We focused on one measure of discriminative model performance, namely timedependent AUC at 10 years of followup according to the KaplanMeier method by Heagerty et al. [33]. Accordingly ΔAUC(10 years) was used as a measure of added predictive performance of the inflammationrelated markers beyond the known predictors.
Evaluation of competing strategies
In the de novo simulation study, the performance of the competing strategies of combining internal validation with imputation was assessed in terms of absolute bias, variance and mean squared error (MSE) of estimated performance criteria as compared to ‘true’ performance, defined as the average performance obtained when the model was fitted on the full (complete) data sets and evaluated on large (n=10,000) independent data sets with same underlying simulated effect sizes. Note that we did not compare (Δ)AUC estimates against the theoretical (Δ)AUC from which effect sizes were derived for simulation (see above), since these are often not achieved with small samples. In the datadriven simulation study, true effects were unknown. There, results of the competing strategies were compared against those from complete data.
Construction of confidence intervals for performance estimates
Jiang et al. [34] proposed a simple concept to estimate confidence intervals for prediction errors in complete data. It is based on the numerical finding that the crossvalidated prediction error asymptotically has the same variability as the apparent error. Thus, they suggest to construct confidence intervals for the prediction error by generating a percentile interval based on resampling for the apparent error and centering this interval at the prediction error. The underlying theory extends to other performance/precision measures [35]. Using the notation of the present manuscript, their proposed procedure follows the steps:

Estimate the prediction error (point estimate) based on crossvalidation (i.e. \(\hat \theta ^{{Train,Test}}\)).

Conduct resampling (they suggest perturbation resampling, where random weights are assigned to the observations in each resampling step; for details we refer to their manuscript): For b=1,…,B, determine the resampling apparent error resulting from the resampled data (i.e. \(\hat \theta ^{{BS(b),BS(b)}}\)). Substract the original apparent error from the resampled one: \(w_{b} = \hat \theta ^{{BS(b),BS(b)}}  \hat \theta ^{{Orig,Orig}}\).

Obtain the α/2 and 1−α/2 percentiles \(\hat \xi _{\alpha /2}\) and \(\hat \xi _{1\alpha /2}\) from the resampling distribution of the w_{ b }, b=1,…,B.

Define the confidence interval for the prediction error as \(\left [\hat \theta ^{{Train,Test}}  \hat \xi _{1\alpha /2}\text {, } \hat \theta ^{{Train,Test}} + \hat \xi _{\alpha /2}\right ]\).
We modified the methodology with regard to several aspects. In step (2), we first used standard nonparametric bootstrapping as described above, and second, allowed for incomplete data by means of one of the combination strategies described above and in Fig. 2. That is, we obtained estimates \(\hat \theta ^{{BS(b,m),BS(b,m)}}, b=1,\ldots,B, m=1,\ldots,M\), by fitting and evaluating the model in each (imputed) BS sample (i.e., in each BS sample that was imputed when strategy ValMI was applied, or in each BS sample drawn from imputed data when strategy MIVal was applied). For each b and m, we defined \(w_{b,m} = \hat \theta ^{{BS(b,m),BS(b,m)}}  \hat \theta ^{{Orig,Orig}}\). In step (3), we obtained the α/2 and 1−α/2 percentiles from the empirical distribution of the w_{b,m}, i.e. across all B×M estimates obtained. In step (4), we centered this interval at the BS 0.632+ estimate (\(\hat \theta ^{0.632+}\)) rather than the CV estimate \(\hat \theta ^{{Train,Test}}\): \(\left [\hat \theta ^{0.632+}  \xi _{1\alpha /2}\text {,}\, \hat \theta ^{0.632+}  \xi _{\alpha /2} \right ]\), with α=0.05. The modified methodology can be integrated with performance estimation using the strategies described above within the same resampling (BS) scheme. For ValMI, we performed B=100 bootstrap draws followed by M=1 imputation; for MI(y)Val, M=100 imputations were conducted followed by B=1 bootstrap draw. For complete data, B=100 was chosen. For comparison, we also constructed confidence intervals for apparent performance based on analytical test concepts, i.e., using DeLong’s test for AUC and ΔAUC. In the presence of missing values (strategies MI and MI(y)), Rubin’s rules were applied to the AUC estimates and variances obtained from DeLong’s test [8].
Software
All calculations were performed using R, version 3.0.1 [36]. Data generation involved use of the R package mvtnorm, version 0.99995 [37]. MICE was performed using the package mice, version 2.17 [6]. Internal validation was performed using custom code. For predictive performance measures, the R packages pROC, version 1.7.3 [38], PredictABEL, version 1.22 [39], and survivalROC, version 1.0.3 [40], were used. Example R code is available in Additional file 2.
Results
Importance of validation and comparative performance of validation strategies
In the de novo simulation experiment, complete and incomplete data were generated with varying data set characteristics, followed by applying the competing combined validation/imputation strategies. For comparison, we also assessed apparent performance, i.e., the performance in the original data in the case of complete data, and the performance estimates pooled using Rubin’s rules from MI in the case of incomplete data. Results are shown in Fig. 3 (for AUC, at n=200, p=10 covariates) and in Additional file 1: Figures S3 to S8 (for other performance measures and choices of parameters).
The apparent performance estimates were generally optimistic – even in the case of large sample size and small number of variables (Additional file 1: Figure S3; n=2000, p=1). Optimism was particularly strongly pronounced for imputed data when the outcome had been included in the imputation models (strategy MI).
Among the investigated ways to correct for optimism, the ordinary optimism correction and the 0.632 estimate tended to achieve less effective optimism control as compared to the BS/SS 0.632+ estimate, the BS/SS OOB estimate and CV estimates. This was most strongly observed in the absence of a true effect and with increasing number of covariates (Fig. 3 and in Additional file 1: Figures S4 to S8).
Comparison of strategies of combining internal validation and multiple imputation
The MIVal strategy, i.e., conducting MI followed by internal validation (i.e., BS, SS, CVK or CVKrep) on the imputed data sets, generally yielded optimistically biased performance estimates and large mean squared errors in almost all settings, and more severely with an increasing number of variables, decreasing sample size, increasing degree of missingness, and decreasing true effect (shown for the AUC in Fig. 4 and in Additional file 1: Figure S9).
MI(y)Val was largely unbiased in the absence of a true effect, but gave pessimistic results when the covariates truely affected the outcome (Fig. 4), largely independent of the number of covariates and the sample size. A likely explanation is that omitting the outcome from the imputation disrupts the correlation structure among covariates and outcome, leading to underestimation of effect sizes. The pessimistic bias became more pronounced with increasing degree of missingness and increasing effect size.
ValMI produced mostly unbiased AUC estimates; however, in the presence of a large number of missing values, a pessimistic bias was observed in the presence of a true underlying effect (Fig. 4). This trend was mostly weaker than for the MI(y)Val strategy and depended also on sample size, number of covariates and true effect size.
Varying other data set characteristics, such as missingness mechanism, outcome class frequencies, correlation among the variables, number of baseline covariates and degree of missingness among baseline covariates, did not greatly influence results (Additional file 1: Figures S10 and S15).
Trends observed for different model performance measures
Although focusing on the AUC as a discrimination measure, the above described trends were largely similar across the model performance measures investigated (Additional file 1: Figures S11 to S21). Of note, biases that were already present in complete data were found to be mirrored, and sometimes augmented, in incomplete data. Examples include the negative bias of ΔAUC (Additional file 1: Figures S13 and S15) and the positive bias of categorical NRI (Additional file 1: Figure S16) in the absence of a true effect, specifically with increasing number of covariates and decreasing sample size. Another example is the pessimistic bias of the Brier score that was most strongly observed for ValMI with increasing degree of missingness, number of covariates and decreasing sample size. Importantly, both ValMI and MI(y)Val strategies generally did not produce (optimistic) bias that was not already (at least to a weaker extent) observed in complete data results.
In terms of calibration, models tended to be miscalibrated in test (OOB) data for most strategies in both complete and incomplete data (Additional file 1: Figures S22 to S27). This trend became worse with decreasing number of covariates and was often observed such that calibration lines were too steep (i.e., intercept <0; slope >1), rendering recalibration of prediction models a desirable step. Although not influencing discriminative test performance, this might improve overall test performance (as measured e.g. by the Brier score).
Extension to a realdata situation
In order to assess how the competing strategies of combining internal validation and MI performed in a realistic situation, we based another simulation experiment on a real data set. In the populationbased MONICA/KORA subcohort, the aim was to assess the ability of blood concentrations of inflammatory markers for predicting allcause mortality over a followup time of 15 years in n=2012 healthy adults. We used the 1258 complete observations as a basis for a datadriven simulation study, where we imposed missingness on these data in a way that reflected the missingness structure in the original incomplete data set (Additional file 1: Figure S1), followed by applying the competing combined validation/imputation strategies to obtain timedependent (change) in AUC.
Results are shown in Fig. 5. Without validation, performance estimates were much higher than those obtained with validation, confirming the importance of validation for assessment of predictive performance. With ordinary optimism correction, performance estimates were still higher than for the other estimates, in line with the assumption that it may achieve insufficient correction for optimism. The lowest values were observed for the OOB, CV3 and CV3rep estimates, suggesting a pessimistic bias, which seemed to be improved by the 0.632+ estimates.
Differences between the strategies of combining validation and imputation were less pronounced, presumably due to the large sample size and small proportion of missing values (7.2 % on average among the inflammationrelated markers). ValMI yielded lower ΔAUC estimates on average as compared to Val on complete data. This was consistent with our observation of a slight pessimism of ValMI in the de novo simulation study in the presence of a true effect, and was even more strongly observed for CV10 and CV10rep. ValMI also appeared more variable as compared to the other strategies. This is likely due to the fact that at the given low proportion of missing values, e.g. performing B=10 BS first followed by M=5 imputations on each yields less distinct data sets than performing M=5 imputations first followed by B=10 random BS runs or performing B=50 BS runs on the complete data.
Choice of number of resamples and number of imputations in practice
We addressed the question of how large the number of resamples B and the number of imputations M should be chosen in practice, for two of the bestperforming strategies, ValMI and MI(y)Val based on bootstrapping with the 0.632+ estimate. Therefore, we repeated the de novo simulation study for selected parameter settings with varying B and M.
For ValMI, we observed a steep decline of variability of performance estimates with increasing B, where as decline was weaker with increasing M (Fig. 6). This is expected, especially in the settings with lower degree of missingness, where the imputed data sets are not expected to differ strongly from each other. At constant total number B·M, the best option seems to be to choose the largest possible value of B (with M=1). This is also not unexpected, given that imputation variability is added on top of resampling variability in each sample.
In contrast, for MI(y)Val, M seemed to be the number that mostly determined variability, with variability decreasing with increasing M even at constant B·M (Fig. 6). Furthermore, variability of performance estimates was generally larger in ValMI as compared to MI(y)Val, even with the least variable combination of B and M at constant total number B·M.
Thus, it is recommendable to choose B and M as large as possible if applying ValMI and MI(y)Val, respectively. An analytic relationship can be utilized in order to assess variability of performance estimates with increasing B and M, respectively: The standard deviation of the mean is generally equal to the population standard deviation divided by the square root of the sample size, given that values are independent. Since the B performance estimates obtained with e.g. ValMI are independent with regard to the BS, we can assume that the following relationship holds:
whereby SD denotes the standard deviation, and \(\hat \theta _{B}\) the performance estimate when B resamples were conducted (and M=1 imputations). Empirical evidence confirms this assumption for both ValMI and MI(y)Val (Additional file 1: Figures S28 and S29). Thus, we provide standard deviation estimates at B=1 and M=1 for various parameter settings in Additional file 1: Tables S2 and S23. This may allow the reader to approximate the standard deviation for their situations at larger values of B or M using Eq. (2) and to choose B or M such that the required accuracy is obtained.
Incomplete future patient data
In the context of building prediction models in the presence of missing values, it has been noted earlier that future patients, to which the prediction model will be applied, might not have complete data for all covariates in the model [13]. To still allow application of the model, the missing values might be imputed using a set of patient data, whereby, notably, the outcome variable is not available. Thus, a relevant question that arises is whether and how predictive performance suffers from missingness in the evaluation data. Therefore, we evaluated models fitted to simulated complete data in large independent data sets with the same underlying simulated effect sizes and varying degrees of missingness, imputed using MI(y). We observed a clear decrease of predictive performance when the proportion of missing values in the test data increased (Additional file 1: Figure S30). This was observed most severely (in absolute terms) with larger true performance.
An approach towards confidence intervals for performance estimates
As an outlook, we considered an approach of constructing resamplingbased confidence intervals for performance estimates that is based on the work by Jiang et al. [34]. Figure 7 shows type 1 error and power for AUC and ΔAUC estimates for the competing strategies. Thereby, type 1 error was defined as the proportion of simulations with true AUC =0.5 or ΔAUC=0, where a test with the null hypothesis AUC =0.5 or ΔAUC=0 was rejected (i.e., confidence interval above 0.5 and 0, respectively). In the presence of a true effect (AUC > 0.5 or ΔAUC>0), this proportion specified power. In a lowdimensional situation (p=1), the nominal type 1 error rate of 5 % was kept on average for all strategies (Fig. 7a, c, e, g). However, at p=10 severely inflated type 1 error rates were observed for the strategies without validation (i.e., based on DeLong’s test) and for the MIVal 0.632+ estimate, while in complete data, ValMI and MI(y)Val, the 0.632+ estimate kept the nominal type 1 error rate (Fig. 7b, d, f, h). As expected, the presence of missing values diminished power, as observed for ValMI as compared to Val on complete data, and to an even stronger extent for MI(y)Val. Together, the proposed approach proposes to be a way of obtaining valid confidence intervals for both ValMI and MI(y)Val 0.632+ estimates without additional computational costs.
Discussion
Using simulated and real data we have compared strategies of combining internal validation with multiple imputation in order to obtain unbiased estimates of various (added) predictive performance measures. Our investigation covered a wide range of data set characteristics, validation strategies and performance measures, and also dealt with practical questions such as the numbers of imputations and bootstrap samples to be chosen in a given data set, and the aspects of incomplete future patient data and the construction of confidence intervals for performance estimates.
Throughout the investigated simulation settings, we observed an optimistic bias for apparent performance estimates, which was insufficiently corrected by ordinary optimism correction and the BS (and SS) 0.632 estimate, whereas the OOB estimate tended to be pessimistic and the 0.632+ tended to provide unbiased estimates. CV estimates were more variable than BS estimates (although this comparison might not be completely fair since the total number of training/test set pairs was not always the same in BS/SS as in CV or CVrep). These trends were similarly observed for complete and incomplete data and are consistent with previous observations for complete data. For instance, Wehberg and Schumacher [41] reported the 0.632+ method to outperform ordinary optimism correction and 0.632, while the OOB estimate was pessimistic. Also, Smith et al. [1] and BragaNeto et al. [42] observed insufficient optimism correction for the ordinary method and the 0.632 estimate, respectively, and both reported increased variability of CV estimates. Another publication focused on AUC estimation and found the BS 0.632+ estimate to be the least biased and variable one among the BS estimates [43].
When we investigated strategies of combining validation with imputation, we observed an optimistic bias for the strategy of imputing first and then resampling on the imputed data (MIVal), whereas imputing training and test sets separately (ValMI) provided largely unbiased and sometimes pessimistic results. The question of in which order bootstrapping and imputation should be combined has been studied before from a theoretical [44] and empirical [12] perspective. In MIVal, all observations, which are later on repeatedly separated into training (BS) and test (OOB) sets, are imputed in one imputation process. Since values are imputed using predictions based on multivariate models including all observations, it is evident that future test observations do not remain completely blind to future training observations. Still, the severity of the expected optimism of the MIVal approach given different data characteristics, validation strategies and performance estimates has not been intensively studied. In practice, both MIVal and ValMI have been applied before [9, 10, 45].
ValMI tended to be pessimistically biased in the presence of a true underlying effect in our and others’ [12] work. Specifically, when sample size is low and number of covariates large, the model overfits the training (BS) part of the data set, resulting in a worse fit to the test (OOB) data. In the presence of missing values, training and test data are imputed separately. It can be assumed that overfitting also occurs at the stage of imputation (where imputation models might become overfitted to the observed data both in the training and in the test set). This may result in a more severe difference in the observed covariateoutcome relationships between training and test data, and consequently worse fit of the model fitted to the training data to the test data, yielding an underestimation of predictive performance that apparently cannot be fully corrected using the 0.632+ estimate.
MI(y)Val produced mostly pessimistic results in the presence of an underlying true effect, mostly independent of sample size and number of covariates. In general MI literature, it is not recommended to omit the outcome from the imputation models [26, 46]. Omitting the outcome equals making the assumption that it is not related with the covariates, as stated by von Hippel [26]. This assumption is wrong in the case of a true underlying effect, resulting in misspecified imputation models, and, in turn, in an underestimation of effect estimates [46]. Of note, the same study reported no difference between the MI and MI(y) methods as far as inference is concerned. To our knowledge, the issue has not been investigated in the context of predictive performance estimation. In their study of ‘incomplete’ CV, Hornung et al. [14] investigated the effect of – amongst other preprocessing steps – imputing the whole data set prior to CV as compared to basing the imputation on the training data only. They used a single imputation method that omitted the outcome, and found only little impact on CV error estimation.
For measures of added predictive performance we made the observation that even in complete data, estimates were sometimes biased in the absence of a true effect. For instance, ΔAUC and categorical NRI were pessimistically and optimistically biased, respectively. The optimistic bias of NRI has lead to critical discussion [47]. It is not unexpected that such bias is not eliminated when the respective validation method is combined with imputation.
Our study focused on treating missing values and deriving reasonable estimates for predictive performance measures in the presence of incomplete data in the model development phase, i.e., in the phase where complete outcome data are available and one aims to derive a prediction model for use in future data.
Our study focused on treating missing values and deriving reasonable estimates for predictive performance measures in the presence of incomplete data in the research stage, i.e. in the situation where data sets with complete outcome data are available from studies/cohorts and one aims to develop a prediction model for use in future patient data (as opposed to the application stage where the model is applied to predict patients’ outcome). Thus, when we evaluated estimates, they were compared against average performance in large complete data sets. An important question is how missing values in future patient data impair the performance of a developed prediction model, and whether such impairment would have to be considered already when developing the model. It has been suggested that data in the research stage should be imputed omitting the outcome from the imputation process, at least in the test sets, to get close to the situation in future realworld clinical data, where no outcome would be available for imputation either [13]. According to this suggestion, the strategy ValMI should be avoided. However, how close a predictive performance estimate obtained through any strategy on the research data approximates the actual performance in future clinical data, depends strongly on the similarity in the proportion (and putatively, in the pattern) of missing values in both situations. Our and others’ [48] results suggest that – irregardless of how missing values in future clinical data are treated – accuracy is lost with increasing missingness in future data at a given proportion of missingness in the research data. We expect the proportion of missing values in future patient data to be lower than that in study data in many cases. Specifically, epidemiological study data are subject to additional missingness attributable to design, sample availability and questionnaire response. Since the precise missingness patterns in both study data and future patient data in clinical practice may vary between studies and the outcome of interest, no general rule can be developed for estimating predictive performance of a model when future patient data are expected to contain missing values.
We propose a simple integrated approach for the construction of confidence intervals for performance estimates. The resulting intervals kept the nominal type 1 error rate for both ValMI and MI(y)Val, although a severe loss in power as compared to complete data could be observed. The chosen approach relies on the numerical finding that prediction error estimates have the same variability as apparent error estimates and thus, bootstrap intervals for apparent error can be centered at prediction error estimate [34]. The strategy has a major computational advantage over alternative strategies of constructing confidence intervals for estimates of prediction error/performance measures that use resampling in order to estimate the distribution of e.g. CV errors [49]. The latter require nesting the whole validation (and imputation) procedure within an outer resampling loop. Other alternatives that do not require a double resampling loop might rely on tests applied to the test data. An example is the median P rule suggested by van de Wiel et al. [50], where a nonparametric test is conducted on the test parts of a subsampling scheme, resulting in a collection of P values of which the median is a valid summary that controls the type 1 error under fairly general conditions. The methodology could be generalized to other (parametric or nonparametric) tests conducted on the test observations, such as DeLong’s test for (Δ)AUC, and extension to incomplete data is possible with the help of Rubin’s combination rules. However, this strategy might lack power, because tests are conducted on the small test sets.
Together, our findings allow the careful formulation of recommendations for practice. First, if one aims to assess predictive performance of a model, validation is of utmost importance to avoid overoptimism. As for complete data, bootstrap with the 0.632+ estimate, turned out to be a preferable validation strategy also in the case of incomplete data. When combining internal validation and MI, one should not impute the full data set including the outcome in the imputation followed by resampling (strategy MIVal) due to its optimistic bias. Instead, we can recommend nesting the MI in the resampling (ValMI) or performing MI first, but without including the outcome variable (MI(y)Val). The number of resamples (B) and imputations (M) should be maximized in ValMI and MI(y)Val, respectively. The choice of exact number of resamples and imputations for a given data set can be guided by the variability data we provide. In many situations and for many performance criteria, ValMI might be preferable, although this choice may also depend on computational capacity, which is lower for MI(y)Val, where variability of the 0.632+ estimate is lower at the same number of resamples and only half the number of imputation runs is required. One should also be aware of (completedata) biases of specific performance criteria, which may be augmented in the presence of missing values. Finally, one possible way of constructing valid confidence intervals for predictive performance estimates may be to center the bootstrap interval of the apparent performance estimate at the predictive performance estimate. This strategy can be easily embedded in the ValMI and MI(y)Val strategies.
Strengths of this study include its comprehensiveness with regard to different data characteristics, validation strategies and performance measures, and the use of both simulated and real data. Our investigation may be extended with regard to several aspects. For instance, we did not vary effect strengths between the covariates. The relationship between effect strengths and missingness in covariates may influence the extent of potential bias in e.g. ValMI. Furthermore, it will be interesting to extend the study on confidence intervals by adopting alternative approaches to incomplete data, with a focus on searching for a strategy that improves power. In addition, one might explore the role of the obtained findings in a higherdimensional situation where variable selection and parameter tuning often requires an inner validation loop. Of note, while in our study results were very similar for BS and SS, in an extended situation involving model selection, or hypothesis tests following [50], SS should be preferred due to known flaws of the BS methodology [51].
Conclusions
In the presence of missing values, our most recommendable strategy to obtain estimates of predictive performance measures is to perform bootstrap for internal validation, with separate imputation of training and test parts and to determine the 0.632+ estimate. For this strategy, at given computational capacity, the number of resamples should be maximized. The strategy allows for the integrated calculation of confidence intervals for the performance estimate.
Abbreviations
 ΔAUC:

Change in AUC
 AUC:

Area under the ROC curve
 BS:

Bootstrap
 CV:

Crossvalidation
 CVK:

Kfold crossvalidation
 CVKrep:

Repeated Kfold crossvalidation
 HDL:

High density lipoprotein
 IDI:

Integrated discrimination improvement
 KORA:

Cooperative health research in the region of Augsburg
 MAR:

Missing at random
 MARblock:

Blockwise missing at random
 MCAR:

Missing completely at random
 MI:

Multiple imputation
 MIVal:

Multiple imputation followed by internal validation
 MI(y):

Multiple imputation without including the outcome in the imputation models
 MI(y)Val:

MI(y) followed by internal validation
 MICE:

Multiple imputation by chained equations
 MONICA:

Monitoring trends and determinants in cardiovascular disease
 NRI:

Net reclassification improvement
 OOB:

Outofbag
 ROC:

Receiveroperating characteristic
 SS:

Subsampling
 Val:

Validation
 ValMI:

Internal validation followed by multiple imputation
References
Smith GCS, Seaman SR, Wood AM, Royston P, White IR. Correcting for optimistic prediction in small data sets. Am J Epidemiol. 2014; 180(3):318–24.
Steyerberg EW, Jr Harrell F, Borsboom GJ, Eijkemans MJ, Vergouwe Y, Habbema JD. Internal validation of predictive models: efficiency of some procedures for logistic regression analysis. J Clin Epidemiol. 2001; 54(8):774–81.
Jr Harrell F, Lee KL, Mark DB. Multivariable prognostic models: issues in developing models, evaluating assumptions and adequacy, and measuring and reducing errors. Stat Med. 1996; 15(4):361–87.
Steyerberg EW, Vickers AJ, Cook NR, Gerds T, Gonen M, Obuchowski N, Pencina MJ, Kattan MW. Assessing the performance of prediction models: a framework for traditional and novel measures. Epidemiology. 2010; 21(1):128–38.
Raessler S, Rubin DB, Zell ER. Incomplete data in epidemiology and medical statistics. Handb Stat. 2008; 27:569–601.
van Buuren S, GroothuisOudshoorn K. mice: Multivariate imputation by chained equations in R. J Stat Softw. 2011; 45:1–67.
van Buuren S, Boshuizen HC, Knook DL. Multiple imputation of missing blood pressure covariates in survival analysis. Stat Med. 1999; 18:681–94.
Rubin DB. Multiple Imputation for Nonresponse in Surveys. New York: John Wiley & Sons; 1987.
Heymans MW, van Buuren S, Knol DL, van Mechelen W, de Vet HCW. Variable selection under multiple imputation using the bootstrap in a prognostic study. BMC Med Res Methodol. 2007; 7:33.
Vergouw D, Heymans MW, Peat GM, Kuijpers T, Croft PR, de Vet HCW, van der Horst HE, van der Windt DAWM. The search for stable prognostic models in multiple imputed data sets. BMC Med Res Methodol. 2010; 10:81.
Vergouwe Y, Royston P, Moons KGM, Altman DG. Development and validation of a prediction model with missing predictor data: a practical approach. J Clin Epidemiol. 2010; 63(2):205–14.
Musoro JZ, Zwinderman AH, Puhan MA, ter Riet G, Geskus RB. Validation of prediction models based on lasso regression with multiply imputed data. BMC Med Res Methodol. 2014; 14:116.
Wood AM, Royston P, White IR. The estimation and use of predictions for the assessment of model performance using large samples with multiply imputed data. Biom J. 2015; 57(4):614–32.
Hornung R, Bernau C, Truntzer C, Wilson R, Stadler T, Boulesteix AL. A measure of the impact of CV incompleteness on prediction error estimation with application to PCA and normalization. BMC Med Res Methodol. 2015; 15:95.
Su JQ, Liu JS. Linear combinations of multiple diagnostic markers. J Am Stat Assoc. 1993; 88(424):1350–5.
Marshall A, Altman DG, Royston P, Holder RL. Comparison of techniques for handling missing covariate data within prognostic modelling studies: a simulation study. BMC Med Res Methodol. 2010; 10:7.
Holle R, Happich M, Lowel H, Wichmann H. KORA – a research platform for population based health research. Gesundheitswesen. 2005; 67:19–25.
Herder C, Baumert J, Zierer A, Roden M, Meisinger C, Karakas M, Chambless L, Rathmann W, Peters A, Koenig W, Thorand B. Immunological and cardiometabolic risk factors in the prediction of type 2 diabetes and coronary events: MONICA/KORA Augsburg casecohort study. PLoS ONE. 2011; 6:19852.
Thorand B, Zierer A, Huth C, Linseisen J, Meisinger C, Roden M, Peters A, Koenig W, Herder C. Effect of serum 25hydroxyvitamin D on risk for type 2 diabetes may be partially mediated by subclinical inflammation: results from the MONICA/KORA Augsburg study. Diabetes Care. 2011; 34(10):2320–2.
Karakas M, Koenig W, Zierer A, Herder C, Rottbauer W, Baumert J, Meisinger C, Thorand B. Myeloperoxidase is associated with incident coronary heart disease independently of traditional risk factors: results from the MONICA/KORA Augsburg study. J Intern Med. 2012; 271(1):43–50.
Raghunathan TE, Lepkowski JM, Hoewyk JV, Solenberger P. A multivariate technique for multiply imputing missing values using a sequence of regression models. Surv Methodol. 2001; 27:85–95.
Yuan Y. Multiple imputation using sas software. J Stat Softw. 2011; 45:1–25.
Efron B, Tibshirani R. Improvement on crossvalidation: the 0.632+ bootstrap method. J Am Stat Assoc. 1997; 92:548–60.
Gerds TA, Cai T, Schumacher M. The performance of risk prediction models. Biom J. 2008; 50(4):457–79.
Efron B. Estimating the error rate of a prediction rule: Some improvements on crossvalidation. J Am Stat Assoc. 1983; 73:555–66.
von Hippel PT. Regression with missing Y’s: an improved method for analyzing multiplyimputed data. Sociol Methodol. 2007; 37:83–117.
Jr Harrell F, Califf RM, Pryor DB, Lee KL, Rosati RA. Evaluating the yield of medical tests. JAMA. 1982; 247(18):2543–6.
Miller ME, Hui SL, Tierney WM. Validation techniques for logistic regression models. Stat Med. 1991; 10(8):1213–26.
Brier G. Verification of forecasts expressed in terms of probability. Mon Weather Rev. 1950; 78:1–3.
Pencina MJ, Sr D’Agostino RB, Jr D’Agostino RB, Vasan RS. Evaluating the added predictive ability of a new marker: From area under the roc curve to reclassification and beyond. Stat Med. 2008; 27:157–72.
Mihaescu R, van Zitteren M, van Hoek M, Sijbrands EJG, Uitterlinden AG, Witteman JCM, Hofman A, Hunink MGM, van Duijn CM, Janssens ACJW. Improvement of risk prediction by genomic profiling: reclassification measures versus the area under the receiver operating characteristic curve. Am J Epidemiol. 2010; 172(3):353–61.
Pencina MJ, Sr D’Agostino RB, Steyerberg EW. Extensions of net reclassification improvement calculations to measure usefulness of new biomarkers. Stat Med. 2011; 30(1):11–21.
Heagerty PJ, Lumley T, Pepe MS. Timedependent roc curves for censored survival data and a diagnostic marker. Biometrics. 2000; 56:337–44.
Jiang B, Zhang X, Cai T. Estimating the confidence interval for prediction errors of support vector machine classifiers. J Mach Learn Res. 2008; 9:521–40.
Uno H, Cai T, Tian L, Wei L. Evaluating prediction rules for tyear survivors with censored regression models. J Am Stat Assoc. 2007; 102(478):527–37.
R Core Team. R: A Language and Environment for Statistical Computing. Vienna: R Foundation for Statistical Computing; 2014. http://www.Rproject.org/. R Foundation for Statistical Computing.
Genz A, Bretz F. Computation of Multivariate Normal and t Probabilities. Lecture Notes in Statistics, Vol. 195. Heidelberg: SpringerVerlag; 2009. ISBN:9783642016882. http://CRAN.Rproject.org/package=mvtnorm.
Robin X, Turck N, Hainard A, Tiberti N, Lisacek F, Sanchez JC, Müller M. pROC: An opensource package for R and S+ to analyze and compare ROC curves. BMC Bioinforma. 2011; 12:77.
Kundu S, Aulchenko YS, Janssens ACJW. PredictABEL: Assessment of Risk Prediction Models.
Heagerty PJ, packaging by Paramita SahaChaudhuri. survivalROC: Timedependent ROC curve estimation from censored survival data. 2013. R package version 1.0.3, http://CRAN.Rproject.org/package=survivalROC.
Wehberg S, Schumacher M. A comparison of nonparametric error rate estimation methods in classification problems. Biom J. 2004; 46(1):35–47.
BragaNeto UM, Dougherty ER. Is crossvalidation valid for smallsample microarray classification?Bioinformatics. 2004; 20(3):374–80.
Sahiner B, Chan HP, Hadjiiski L. Classifier performance prediction for computeraided diagnosis using a limited dataset. Med Phys. 2008; 35(4):1559–70.
Shao J, Sitter RR. Bootstrap for imputed survey data. J Am Stat Assoc. 1996; 91(435):1278–88.
Siersma V, Johansen C. The use of the bootstrap in the analysis of casecontrol studies with missing data. 2004. Technical report.
Moons KGM, Donders RART, Stijnen T, Jr Harrell FE. Using the outcome for imputation of missing predictor values was preferred. J Clin Epidemiol. 2006; 59(10):1092–101.
Pepe MS, Fan J, Feng Z, Gerds T, Hilden J. The net reclassification index (NRI): a misleading measure of prediction improvement even with independent test data sets. Stat Biosci. 2015; 7(2):282–95.
Zhang Q, Rahman A, D’este C. Impute vs. ignore: Missing values for prediction. In: Neural Networks (IJCNN), The 2013 International Joint Conference On. IEEE: 2013. p. 1–8. http://ieeexplore.ieee.org/document/6707014/.
Jiang W, Varma S, Simon R. Calculating confidence intervals for prediction error in microarray classification using resampling. Stat Appl Genet Mol Biol. 2008; 7(1):8.
van de Wiel MA, Berkhof J, van Wieringen WN. Testing the prediction error difference between 2 predictors. Biostatistics. 2009; 10(3):550–60.
Janitza S, Binder H, Boulesteix AL. Pitfalls of hypothesis tests and model selection on bootstrap samples: Causes and consequences in biometrical applications. Biom J. 2015; 58(3):447–73.
Acknowledgements
We thank all MONICA/KORA study participants and all members of the field staff in Augsburg who planned and conducted the study. We thank Annette Peters, head of the KORA platform, for providing the data, and Andrea Schneider for excellent technical support. We thank the involved cooperation partners Wolfgang Koenig (University of Ulm Medical Center, Ulm, Germany) and Christian Herder (German Diabetes Center, Düsseldorf, Germany) for permission to use the MONICA/KORA subcohort data for the present analyses.
Funding
The MONICA/KORA research platform and the KORA Augsburg studies are financed by the Helmholtz Zentrum München  German Research Center for Environmental Health, which is funded by the German Federal Ministry of Education and Research (BMBF) and by the State of Bavaria. Laboratory measurements in the MONICA/KORA casecohort study were funded through research grants from the German Research Foundation (DFG) (TH784/21 and TH784/22) and additional funds provided by the University of Ulm, the German Diabetes Center, Düsseldorf, the Federal Ministry of Health and the Ministry of Innovation, Science, Research and Technology of the state North RhineWestphalia). Astrid Zierer was supported by the Else KrönerFreseniusStiftung. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Availability of data and materials
MONICA/KORA subcohort data are available upon request through the application tool KORA.PASST (http:/epi.helmholtzmuenchen.de). R code for reproduction of the simulation study is available in Additional file 2.
Authors’ contributions
SW, AB and MW devised the basic idea for the manuscript. SW performed the statistical analyses with contributions from AB, AZ, BT and MW. BT and AZ contributed the MONICA/KORA data. SW, AB and MW wrote the manuscript. AZ and BT revised the manuscript for important intellectual content. All authors read and approved the final manuscript.
Competing interests
The authors declare that they have no competing interests.
Ethics approval and consent to participate
Written informed consent was obtained from all MONICA/KORA participants and the studies were reviewed and approved by the local ethics committee (Bayerische Landesaerztekammer).
Author information
Authors and Affiliations
Corresponding author
Additional information
The original version of this article was revised. The spelling of author Mark A. van de Wiel’s name was corrected
An erratum to this article is available at http://dx.doi.org/10.1186/s1287401602717.
Additional files
Additional file 1
Supplementary Figures and Tables. Figure S1. Imposing missingness into complete observations from the application data set. Figure S2. Visualization of internal validation strategies in complete data. Figure S3. Simulation distribution of AUC estimates obtained by different strategies at large sample size (n=2000) and p=1 covariate. Figure S4. Simulation distribution of AUC estimates obtained by different strategies at large sample size (n=2000) and p=1 covariate. Figure S5. Simulation distribution of ΔAUC estimates obtained by different strategies. Figure S6. Simulation distribution of categorical NRI estimates obtained by different strategies. Figure S7. Simulation distribution of continuous NRI estimates obtained by different strategies. Figure S8. Simulation distribution of IDI estimates obtained by different strategies. Figure S9. Mean squared error of AUC estimates obtained by different strategies based on bootstrapping. Figure S10. Bias of AUC estimates obtained by different strategies based on bootstrapping – Influence of further data characteristics. Figure S11. Bias of Brier score estimates obtained by different strategies based on bootstrapping. Figure S12. Mean squared error of Brier score estimates obtained by different strategies based on bootstrapping. Figure S13. Bias of ΔAUC estimates obtained by different strategies based on bootstrapping. Figure S14. Mean squared error of ΔAUC estimates obtained by different strategies based on bootstrapping. Figure S15. Bias of ΔAUC estimates obtained by different strategies based on bootstrapping – Influence of further data characteristics. Figure S16. Bias of categorical net reclassification improvement (NRI) estimates obtained by different strategies based on bootstrapping. Figure S17. Mean squared error of categorical net reclassification improvement (NRI) estimates obtained by different strategies based on bootstrapping. Figure S18. Bias of continuous net reclassification improvement (NRI) estimates obtained by different strategies based on bootstrapping. Figure S19. Mean squared error of continuous net reclassification improvement (NRI) estimates obtained by different strategies based on bootstrapping. Figure S20. Bias of continuous integrated discrimination improvement (IDI) estimates obtained by different strategies based on bootstrapping. Figure S21. Mean squared error of integrated discrimination improvement (IDI) estimates obtained by different strategies based on bootstrapping. Figure S22. Simulation distribution of calibration intercept estimates obtained by different strategies. Figure S23. Bias of calibration intercept estimates obtained by different strategies based on bootstrapping. Figure S24. Mean squared error of calibration intercept estimates obtained by different strategies based on bootstrapping. Figure S25. Simulation distribution of calibration slope estimates obtained by different strategies. Figure S26. Bias of calibration slope estimates obtained by different strategies based on bootstrapping. Figure S27. Mean squared error of calibration slope estimates obtained by different strategies based on bootstrapping. Figure S28. Relationship of standard deviations of performance estimates with varying number of resamples and imputations. Figure S29. Standard deviation of performance estimates at varying number of resamples and imputations. Figure S30. Impairment of performance evaluation through missing values in the test data. Table S1. Descriptive information of phenotypic and inflammationrelated markers from the MONICA/KORA subcohort. Table S2. Standard deviation of performance estimates obtained with ValMI at one resample (B=1) and one imputation (M=1). Table S3. Standard deviation of performance estimates obtained with MI(y)Val at one imputation (M=1) and one resample (B=1). (PDF 1823 kb)
Additional file 2
R code. Example_R_Code.r Example R code showing how to apply functions. gen.data.r R functions for generation of data and imposing missingness. do.resampling.r R functions for performing internal validation and multiple imputation. get.performance.r R functions to do the modeling and obtain performance estimates. do.CIs.r R functions to obtain point and confidence interval estimates. (ZIP 20 kb)
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
About this article
Cite this article
Wahl, S., Boulesteix, AL., Zierer, A. et al. Assessment of predictive performance in incomplete data by combining internal validation and multiple imputation. BMC Med Res Methodol 16, 144 (2016). https://doi.org/10.1186/s1287401602397
Received:
Accepted:
Published:
DOI: https://doi.org/10.1186/s1287401602397
Keywords
 Missing values
 Incomplete data
 Prediction model
 Predictive performance
 Bootstrap
 Internal validation
 Resampling
 Crossvalidation
 Multiple imputation
 MICE