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A threshold method for immunological correlates of protection
 Xuan Chen^{1},
 Fabrice Bailleux^{2},
 Kamal Desai^{3}Email author,
 Li Qin^{4, 5} and
 Andrew J Dunning^{6}
https://doi.org/10.1186/147122881329
© Chen et al.; licensee BioMed Central Ltd. 2013
Received: 16 September 2012
Accepted: 19 February 2013
Published: 1 March 2013
The Erratum to this article has been published in BMC Medical Research Methodology 2017 17:19
Abstract
Background
Immunological correlates of protection are biological markers such as diseasespecific antibodies which correlate with protection against disease and which are measurable with immunological assays. It is common in vaccine research and in setting immunization policy to rely on threshold values for the correlate where the accepted threshold differentiates between individuals who are considered to be protected against disease and those who are susceptible. Examples where thresholds are used include development of a new generation 13valent pneumococcal conjugate vaccine which was required in clinical trials to meet accepted thresholds for the older 7valent vaccine, and public health decision making on vaccination policy based on longterm maintenance of protective thresholds for Hepatitis A, rubella, measles, Japanese encephalitis and others. Despite widespread use of such thresholds in vaccine policy and research, few statistical approaches have been formally developed which specifically incorporate a threshold parameter in order to estimate the value of the protective threshold from data.
Methods
We propose a 3parameter statistical model called the a:b model which incorporates parameters for a threshold and constant but different infection probabilities below and above the threshold estimated using profile likelihood or least squares methods. Evaluation of the estimated threshold can be performed by a significance test for the existence of a threshold using a modified likelihood ratio test which follows a chisquared distribution with 3 degrees of freedom, and confidence intervals for the threshold can be obtained by bootstrapping. The model also permits assessment of relative risk of infection in patients achieving the threshold or not. Goodnessoffit of the a:b model may be assessed using the HosmerLemeshow approach. The model is applied to 15 datasets from published clinical trials on pertussis, respiratory syncytial virus and varicella.
Results
Highly significant thresholds with pvalues less than 0.01 were found for 13 of the 15 datasets. Considerable variability was seen in the widths of confidence intervals. Relative risks indicated around 70% or better protection in 11 datasets and relevance of the estimated threshold to imply strong protection. Goodnessoffit was generally acceptable.
Conclusions
The a:b model offers a formal statistical method of estimation of thresholds differentiating susceptible from protected individuals which has previously depended on putative statements based on visual inspection of data.
Keywords
Background
Immunological correlates of protection are measurable and specific biological markers which correlate with protection against disease caused by an infectious pathogen. The markers used are most often pathogenspecific neutralizing antibodies whose concentration can be measured with biological assays [1]. Researchers and agencies responsible for immunization recommendations, such as the US Advisory Committee for Immunization Practices and the World Health Organization, rely on established threshold values for the immunological correlate of protection where the accepted threshold differentiates between individuals who are considered to be immunologically protected against disease and those who are susceptible [2, 3]. When it is strongly correlated with protection with a recognized threshold, it can be called an absolute correlate [4].
Uses for the established threshold for a correlate of protection are numerous. For instance, where the correlate has been established for a vaccine that has already demonstrated clinical efficacy against disease, the correlate simplifies study of the vaccine in new populations, age or riskgroups by permitting clinical trials to be conducted with immunogenicity endpoints and avoiding largescale efficacy trials. The US Food and Drug Administration (FDA) offers accelerated approval when there is a correlate (FDA prefers the term “surrogate”) that is considered “reasonably likely” to predict clinical benefits [5]. Other uses include the study of immunogenicity for coadministration with other vaccines, comparisons of combination vaccines to individual component vaccines and assessment of the duration of protection. The established correlate of protection also permits comparisons of new generation vaccines to older ones. For completely novel vaccines, the demonstration of a candidate immunologic correlate is becoming a secondary yet fundamental objective in clinical trials and epidemiological studies. This is encouraged by agencies such as the US FDA Center for Biologics Evaluation and Research and is one of the Grand Challenges in Global Health [6]. Thus the accurate identification of protective threshold levels clearly has important implications for the licensure of vaccines and for immunization policy.
Research in correlates of protection is multidisciplinary. As a consequence, terminology used has been inconsistent and sometimes confusing. There have been recent efforts to harmonize the terminology employed and to link this to a hierarchy of statistical evidence for the demonstration of a correlate [4, 7, 8]. In addition the terminology has been further refined by introducing the terms mechanistic and nonmechanistic to address whether the correlate of protection is causal or not [9]. We will here for convenience use the term ‘correlate of protection’ in the broadest sense, to include immunological assays that have been consistently shown to correlate with risk of disease, assays that have been shown to be causally associated with protection, or specific threshold values of assays which have been accepted or proposed as differentiating susceptible from protected individuals. We also use the term ‘protective threshold’ to refer to an assay value for the correlate that distinguishes protected and unprotected individuals when the relationship between the correlate and protection can be reliably and usefully summarized with a single threshold value. However, individual variability means that at any threshold value some above will be susceptible and some below protected, and ‘protective threshold’ is not intended to imply any particular level of protection, and specifically is not intended to imply complete protection or ‘sterile immunity’. ‘Assay value’ and ‘titer’ are used interchangeably, according to context. A general opinion is emerging that improvement in statistical methods is needed [10, 11] for identifying correlates of protection, but opinions vary on the appropriate statistical methodology. Methods and study designs have varied historically and across disease areas resulting in different standards of data quality and statistical methods to establish correlates of protection and their threshold values.
For older vaccines, the protective immunological thresholds have often been determined based on observational data, which was sometimes conveniently available or opportunistic. For example, Björkholm et al. measured diphtheria antitoxin titers in 44 individuals admitted to hospital during a diphtheria epidemic among alcoholics in Sweden and observed that 7 of 10 patients who had diphtheria antitoxin titers < 0.01 IU/ml died or showed neurological complications, whereas 33 out of 34 diphtheria carriers with antitoxin titers ≥ 0.16 IU/ml remained symptomfree [12]. Further in vitro studies suggested that titers between 0.01 and 0.09 IU/ml may be regarded as giving basic immunity, whereas a higher titer of 0.1 IU/ml was considered fully protective [13].
When an outbreak of measles occurred among students in a dormitory at Boston University, Chen et al. obtained permission to assay samples of blood donations made shortly before the start of the outbreak and compared their antibody concentrations with the occurrences of measles [14]. Of 9 donors with detectable preexposure plaque reduction neutralization titer less than or equal to 120, 8 met the clinical criteria for measles compared with none of 71 with preexposure titers greater than 120. Similarly, Neumann collected sera from 238 high school students on Prince Edward Island before a measles epidemic sweeping the rest of Canada reached the island to compare infection rates by titer [15].
An early study by Goldschneider et al. established a protective threshold for meningococcal C disease based on serum bactericidal assay [16]. American army recruits provided blood samples for assaying at the start of basic training, and disease occurred in only 1% of individuals who had titers greater than 4 of SBA at recruitment compared to 22% of those who had less than 4. This was further confirmed by a population study that demonstrated an inverse relationship between disease incidence and the presence of SBA titers.
These early studies and others [17] selected protective thresholds based on inspection of disease rates observed in discrete intervals of assay values with confidence limits never reported. Siber provides an indepth discussion of this approach [18] and introduces the idea of titerspecific degrees of protection.
For newer vaccines, clinical trials or observational studies specifically incorporate immunological data collection to identify potential thresholds, and statistical approaches have accordingly been developed for this purpose. For instance, in the ChangKohberger method data from three doubleblind controlled trials in Northern Californian, American Indian and South African infants were pooled in a metaanalysis to derive a protective threshold of 0.35 μg/ml for anticapsular antibodies for a 7valent pneumococcal conjugate vaccine against invasive pneumococcal disease [19, 20]. The statistical method equates relative risk of invasive pneumococcal disease between vaccine and control groups to the relative risk of having antibody concentration below the protective threshold, and the protective threshold is then found from cumulative distribution curves of the antibody concentrations of the vaccinated group and the control groups. The threshold has been endorsed by a WHO Working Group and has subsequently been used to develop and license a newer generation 13valent vaccine [21].
It was essentially this same method that was employed by Andrews et al. to derive a threshold for a correlate of protection following meningococcal C vaccination [22]. The two modern examples for pneumococcal and meningococcal C vaccines that employed the ChangKohberger method, however, required an estimate of vaccine efficacy based on a clinical endpoint before the method could be used.
Few other statistical methods exist for identifying a threshold. The idea of estimating separate disease probabilities a and b below and above a threshold has been proposed by Siber et al. but no actual model was developed to estimate the threshold [20].
Other statistical approaches have focused on continuous models, which do not explicitly model a threshold. Logistic regression has frequently been used [23–28]; other continuous models have included proportional hazards [29] and Bayesian generalized linear models [30]. Chan compared Weibull, lognormal, loglogistic and piecewise exponential models applied to varicella data [31]. A limitation of such models is that they cannot separate exposure to disease from protection against disease given exposure, the latter being the relationship of interest. A scaled logit model which separates exposure and protection where protection is a continuous function of assay value has been proposed [32]. The scaled logit model was illustrated with data from the German pertussis efficacy trial data [27] and has been used to describe the relationship between influenza assay titers and protection against influenza [33–35]. However, these approaches do not explicitly allow identification of a single threshold value.
Thus despite the fundamental reliance on thresholds in vaccine science and immunization policy, previous statistical models have not specifically incorporated a threshold parameter for estimation or testing. In this paper, we propose a statistical approach based on the suggestion in Siber et al. [20] for estimating and testing the threshold of an immunologic correlate by incorporating a threshold parameter, which is estimable by profile likelihood or least squares methods and can be tested based on a modified likelihood approach. The model does not require prior vaccination history to estimate the threshold and is therefore applicable to observational as well as randomized trial data. In addition to the threshold parameter the model contains two parameters for constant but different infection probabilities below and above the threshold and can be viewed as a stepshaped function where the step corresponds to the threshold. The model will be referred to as the a:b model.
Methods
Model specification and fitting
To fit the models, closed form expressions may be derived by maximum likelihood or least squares for estimators of the parameters a, b but not for τ. The estimators for a, b remain as functions of τ. Following the profile likelihood or least squares approach, the optimal value of τ may be found by proceeding through the candidate values, estimating the other parameters and the likelihood or sum of squared errors at each value. The value of τ that maximizes the likelihood or minimizes the sum of squares is the estimate for τ. The derivation of the least squares and maximum likelihood estimators of a, b is shown in the Additional file 1.
A previous method which seeks to identify a cut point is the maximal chisquare proposed by Miller [36]. Here a continuous variable which is predictive of a clinical outcome is dichotomized using a cut point with cases and noncases displayed in a 2×2 table. The optimal cut point corresponds to the maximal chisquare associated with the 2×2 table. It can be shown that the estimated threshold τ selected by least squares in the a:b model corresponds to the optimal cut point obtained via the maximal chisquare method; a proof is given in the Additional file 1.
Testing for the existence of a threshold
Simulations performed under the null hypothesis of no existence of threshold showed that under this hypothesis the distribution of D’ may be approximated by a chisquared distribution with 3 degrees of freedom; thus D’ may be compared to a chisquared distribution with 3 degrees of freedom for testing the null hypothesis of no threshold. The test is an unconditional significance test of the step function represented by τ, a, b compared to a constant probability of infection.
Confidence interval for the threshold value
Confidence intervals for the threshold value may be calculated by nonparametric bootstrapping following standard methods [37]. Datasets were resampled 5000 times with replacement, and the lower and upper limits of the 95% confidence interval for the threshold were based on the 2.5 and the 97.5 percentiles of the estimates of τ from each resampling.
Goodnessoffit
Residuals defined by the differences between the observed dichotomous outcomes and the modeled probability of disease as in the a:b model are not normally distributed and hence goodnessoffit methods relying on normality are inappropriate. Although Pearson and Chisquared deviance residuals may be used for dichotomous outcomes, when the number of discrete values of the model predictors is large, such as for a continuous predictor like titers, their distributions are not well approximated by chisquared distributions since the degrees of freedom increases with the number of discrete values. In such circumstances Hosmer and Lemeshow propose an approach in which the observed predictors are grouped into 10 groups defined by the deciles of the ordered predictors, and goodnessoffit is estimated by the squared difference between observed and predicted infection rates in each group [38].
where g indexes groups 1,…,10, y _{ .g } is the observed number of cases in group g, m _{ g } is the number of subjects in group g, and ${\widehat{\pi}}_{g}$ is the predicted disease probability in the group, i.e. $\widehat{a}$ or $\widehat{b}$ (or a weighted average if the group includes the threshold). Simulations show C to follow a chisquared distribution with 8 degrees of freedom when the model is true, so the goodnessoffit may be quantified by the probability in the upper tail of this distribution. The test assesses whether the step function represented by the a:b model is an appropriate representation of infection or whether another relationship such as a gradual one between titer and infection might be more likely than a stepped relationship.
Relative risk
The relative risk of disease above and below the threshold may be a more readily interpretable measure of the relevance of a fitted threshold. Note that relative risk is not suitable as a criteria for selecting a value of τ, since for different candidate values for τ the relative risk declines from approximately 0.5 at low assay values to near 0 at high values. However, having selected τ, the relative risk quantifies the difference between those above the threshold and those below in terms of the outcome of interest, namely probability of disease. The relative risk is estimated by $\widehat{b}/\widehat{a}\phantom{\rule{0.5em}{0ex}}$. An approximate 95% confidence interval for the relative risk (conditional on the estimated value of τ) can be obtained by parametric bootstrapping.
SAS statistical software was used for all analysis.
Datasets
The a:b model was applied to 15 datasets from four studies. Briefly the datasets are:

German pertussis datasets: eight assays for IgG or IgA antibodies against pertussis toxin (PT), pertactin (PRN), filamentous hemagluttinin (FHA) and fimbriae (FIM) and occurrence of 44 cases of disease in 1994 subjects from a substudy of a pertussis vaccine efficacy trial conducted in Germany between 1991 and 1994 [27]. IgG antibodies are a humoral immune response whereas IgA antibodies are responses at mucosal sites.

Piedra/respiratory syncytial virus (RSV) datasets: assays for antibody to RSV/A and RSV/B among subjects presenting with acute respiratory symptoms at a hospital in Texas, and subsequent disease confirmation in 34 of 175 subjects [26].

White/varicella dataset: varicella glycoprotein assay for children vaccinated with varicella vaccine in clinical trials conducted between 1987 and 1989, and disease occurrence in 79 of 3459 subjects in 12 months of follow up [17].

Swedish pertussis datasets: four assays (IgG antibodies for PT, PRN, FHA, FIM) from subjects exposed to pertussis by another household member and the subsequent development of disease in 92 of 209 subjects, from a substudy of a vaccine efficacy trial conducted in Sweden between 1992 and 1995 [28].
Results
Threshold estimates, statistical significance and confidence intervals
Correlate of protection threshold values $\widehat{\tau}$ estimated by a:b model and evaluation criteria for 15 immunological datasets on pertussis, RSV and varicella
Dataset (cases of disease: subjects)  $\widehat{\tau}$ by profile likelihood (95% CI)  $\widehat{\tau}$ by leastsquares (95% CI)  pvalue, test for threshold^{†}  pvalue, goodnessoffit^{†}  Relative risk^{†}(95% CI) 

German pertussis FHA IgG (44:1988)  1.995 (1.185;18.13)  1.995 (0.990;2.025)  6.0×10^{–11}  0.114  0.093 (0.030;0.183) 
German pertussis PT IgG (44:1987)  1.385 (0.965;1.390)  1.385 (0.755;1.390)  3.2×10^{–11}  0.798  0.055 (0.000;0.133) 
German pertussis PRN IgG (44:1992)  13.165 (1.375;29.31)  7.665 (0.855;13.17)  4.1×10^{–9}  0.615  0.052 (0.000;0.141) 
German pertussis FIM IgG (44:1986)  0.315 (0.305;4.500)  0.315 (0.215;0.540)  9.1×10^{–10}  0.284  0.111 (0.040;0.216) 
German pertussis FHA IgA (44:1932)  0.385 (0.305;1.960)  0.385 (0.315;1.960)  0.344  0.742  0.501 (0.267;1.237) 
German pertussis PT IgA (44:1933)  1.785 (0.475;3.179)  1.785 (0.415;4.064)  0.497  0.502  0.554 (0.181;1.120) 
German pertussis PRN IgA (44:1968)  2.505 (0.760;2.510)  2.505 (0.485;2.510)  1.4×10^{–3}  0.346  0.000 (  ;  ) 
German pertussis FIM IgA (44:1994)  3.385 (1.565;3.830)  1.575 (1.030;3.825)  1.3×10^{–4}  0.159  0.176 (0.037;0.375) 
Piedra RSV/A (34:175)  76.109 (5.657;608.9)  76.109 (4.757;215.3)  2.0×10^{–3}  0.918  0.308 (0.163;0.544) 
Piedra RSV/B (34:175)  107.635 (8.000;1722)  107.635 (5.657;861.1)  2.7×10^{–3}  0.546  0.305 (0.169;0.548) 
White/Varicella (79:3459)  5.011 (2.584;5.011)  2.584 (1.311;5.011)  <1.0×10^{–12}  0.085  0.098 (0.053;0.163) 
Swedish pertussis FHA IgG (92:209)  1.414 (0.707;6.481)  1.414 (0.707;6.481)  3.5×10^{–4}  0.966  0.508 (0.358;0.687) 
Swedish pertussis PT IgG (92:209)  5.477 (1.414;15.49)  5.477 (1.414;10.49)  3.0×10^{–4}  0.999  0.391 (0.199;0.614) 
Swedish pertussis PRN IgG (92:209)  5.950 (2.298;15.92)  5.950 (1.497;6.380)  5.1×10^{–10}  0.921  0.130 (0.030;0.270) 
Swedish pertussis FIM IgG (92:209)  7.650 (1.249;7.846)  4.225 (1.249;7.846)  2.2×10^{–09}  0.781  0.177 (0.056;0.325) 
For 12 of 15 datasets least squares and profile likelihood estimates of τ were the same while in the other 3 datasets (German pertussis PRN IgG, German pertussis FIM IgA, White/varicella) the least squares estimate was lower than the profile likelihood estimate.
Thirteen of 15 thresholds found by the model were highly statistically significant by the modified likelihood ratio test with pvalues <0.01, while two German pertussis datasets for FHA IgA and PT IgA were not significant at the 0.05 level.
There was considerable variability in the widths of the 95% confidence intervals when considered relative to the range of the titers (Figure 1). In one instance, the German pertussis PT IgG data, the confidence interval was notably narrow; in the cases of the RSV/A and RSV/B datasets, the confidence intervals spanned a large proportion of the range of the titers. When fitted by profile likelihood, the point estimate of the threshold for German Pertussis PT IgG, PRN IgA, FIM IgA, White/varicella and Swedish Pertussis FIM datasets was close to the upper limit of the 95% CI and close to the lower limit for the German pertussis FIM IgG dataset. A similar pattern was seen for some datasets when fitted by least squares.
The upper and lower limits of the confidence intervals found by profile likelihood were often found to be greater than by least squares.
Goodnessoffit
Using the adhoc criterion that a goodnessoffit pvalue less than 0.20 represents a poor fit to the data, we found that the a:b model did not fit well to three datasets: White/varicella, German pertussis FHA IgG and German pertussis FIM IgA. Visual inspection of the plots in Figure 1 would suggest that protection against varicella follows a gradually increasing protection rate by titer value rather than a stepwise relationship, explaining the poor fit in this case. The German pertussis FHA IgG and FIM IgA appear to follow a similar gradual protection relationship. Another correlate of protection which may not be well described by the a:b model based on visual inspection of plots is RSV/B, but this was associated with a goodnessoffit pvalue of 0.546. Apart from RSV/B, all other datasets which were associated with goodnessoffit pvalues >0.20 could be visually confirmed to fit the stepwise shape of the a:b model.
Relative risk
The relative risk estimate is dependent on the estimated threshold, and offers an interpretation which is more familiar to the epidemiologist. The relative risk of disease above the threshold compared to below ranged from 0 to 0.554 among the fifteen datasets. Except for 3 relative risks with values near 0.5 and one near 0.4, all other relative risks took values near 0.3 or less implying protection of 70% or better. Thus, in most cases, the estimated threshold corresponds with the notion of an absolute correlate to offer a high degree of protection.
Discussion
Despite the central importance of threshold values in vaccines research and immunization policy, only the ChangKohberger method [19, 20] has been previously proposed to estimate thresholds from assay values and disease occurrence data, but its estimation requires information on vaccinated and unvaccinated groups. The a:b method provides a reliable, readily applicable method for finding a threshold for paired data of the form {y _{ i },t _{ i }} for which previous models and associated statistical testing were limited. The a:b model provides the same estimate as the maximal chisquare method [35] when least squares estimation is used.
The statistical criteria available for the evaluation of a threshold estimated by the a:b model are confidence interval width and location, goodness of fit, significance testing and relative risk. A number of factors are likely to influence the width of confidence intervals, including the presence of a clear, high step in the data and the number of subjects and cases of disease in the dataset. Further, bootstrap confidence intervals based on the candidate values of tau are affected by the density of distinct observed assay values in the region of the threshold. This is a data limitation arising from the assay technique which generates discrete rather than continuous titer values, with lower densities (fewer distinct assay values) tending to produce wider confidence intervals and higher densities allowing the possibility of smaller confidence intervals. The location of threshold point estimates and upper and lower confidence limits in some datasets suggested that profile likelihood estimates may be higher and therefore more conservative, requiring higher antibody titers to be achieved to conclude protection, compared to least squares estimates.
Goodnessoffit pvalue in some instances was clearly consistent with the bar plots of the binned data while in other cases this was less so, possibly due to discreteness in the data resulting from small numbers of cases of disease. Visual inspection of graphical representations of the data might routinely supplement statistical assessments.
Because the estimated threshold itself does not imply the degree of protection, relative risk aids in its interpretation. If a threshold is to separate susceptible from protected individuals, relative risk may be seen as a measure of the degree of protection and can be employed as one of the criteria for assessing the relevance of an estimated threshold in addition to the pvalue from the test for significance. For example, the Swedish pertussis FHA IgG result produced a pvalue of 3.5×10^{4} but a relative risk of 0.508, implying around 50% reduction in risk, which may question the acceptability of the threshold as higher protection is generally expected in vaccine preventable disease.
Ideally, all assessment criteria would provide consistent results in support of a threshold. However, instances were noted where other conclusions might be warranted even though some statistical assessments were promising. For example, for the White/varicella data, there is a small confidence interval for the threshold, the pvalue for the threshold is highly significant and the relative risk acceptable (close to 0.1) but the goodnessoffit is poor (p = 0.085). It was found that that this data is better fitted by a continuous scaledlogit model (p for goodnessoffit = 0.999), suggesting that a relative rather than absolute threshold may be appropriate.
The threshold in the a:b model is the titre value that best separates the sample of patients into two groups with different but constant infection rates, but this does not require the ‘protected’ group to have a specified low probability of infection. It is therefore possible that the protected group defined by the estimated threshold has a high probability of infection, like 20% in the pertussis PT IgG example, which could be deemed to be unacceptably high if one’s definition of a threshold requires low risk of infection. Therefore, an additional criterion that sets a maximally acceptable probability of infection amongst the protected group could be considered in addition to statistical tests when evaluating thresholds.
Although definitions of thresholds may differ, it is encouraging to note that others’ published estimates of thresholds for these same datasets are not dissimilar to estimates from the a:b model, suggesting consistency with others’ notion of an acceptable threshold. For instance, a previous analysis of the White/varicella data identified a gp ELISA titer of 5 U/mL to indicate protection, which is now reported to be an ‘approximate correlate of protection’ for varicella vaccines [39]. The estimate was consistent with our profile likelihood estimate of the threshold of 5.011 (95% CI; 2.584; 5.011). For the Swedish pertussis data, a putative threshold value of 5 units/mL for PRN, FIM and PT were found to be associated with high protection [28]; subjects having all three had even higher protection. However, while the authors applied the same putative threshold to all 3 pertussis components, we estimated different values for each: 5.477 (95% CI; 1.414;15.49) for PT, 5.950 (95% CI; 2.298;15.92) for PRN and 7.650 (95% CI; 1.249;7.846) for FIM. For the German pertussis data, a regression tree approach found that a threshold value of 7 units/mL for PRN IgG was most predictive of protection [23]. We estimated a threshold of 13.165 (95% CI; 1.375;29.31) with profile likelihood and 7.665 (95% CI; 0.855;13.17) using least squares. Amongst the subset of subjects achieving 7 units/mL for PRN, those who had 66 units/mL of PT IgG had even greater protection. Our estimated threshold for PT IgG using profile likelihood was 1.385 (95% CI; 0.965;1.390), but this figure is not comparable to the previous figure of 66 unit/mL which should be interpreted as a conditional threshold given that protective PRN levels are achieved.
Because the a:b model assumes constant rates of infection on each side of the threshold, which may be a strong assumption, we considered in supplementary analyses more flexible models which allowed linear, quadratic or logistic relationships on either side of the threshold. However, these models did not produce fits corresponding with the expectations of a correlate of protection. For instance, a stepdown of infection rate at the threshold value and nonincreasing rates of infection on either side of the threshold were not always observed. The a:b model was always consistent with these expectations. In addition, visual examination of the profile likelihood for these other models did not show sharp peaks corresponding to the optimal threshold value, and were associated with wider confidence intervals resulting in greater uncertainty of the threshold value. In general these more flexible models could not be relied upon to consistently find a threshold which could be said to differentiate protected from susceptible individuals.
The a:b model presented here does not require vaccination information to estimate a threshold. While this is an advantage, it is also a weakness given that the a:b model can provide only the first level of information in the hierarchy of evidence to demonstrate a statistical correlate of vaccine efficacy in the framework described by Qin et al. [7]. To provide a higher level of evidence, the a:b model could be developed to include a vaccination parameter and an associated test. Also, further development could allow for multiple cocorrelates in which two or three threshold values are estimated simultaneously. This could have application to diseases like pertussis where more than one antigen is necessary for the fullest protection or for new vaccines that protect against multiple serotypes of a disease, such as pneumococcal infection or dengue. Further research might also compare different statistical models for correlates of protection – the a:b model, the method of Chang and Kohberger [19–21], the scaled logit model [32–35], a linear trend model and logistic regression – and the conclusions reached by each for levels of protection.
In order to investigate correlates of protection and thresholds, there are also clinical and immunological considerations. A correlate must include a clearly defined clinical endpoint, whether protection is afforded against infection, disease, severe disease, infectiousness, carriage or other condition. For instance, it is thought that protection against pneumococcal infection requires progressively lower thresholds for protection against pneumococcal carriage, otitis media, pneumonia and invasive pneumococcal infection [40]. Similarly, standardized laboratory assays and tests for disease case confirmation are also needed but not always feasible, which can potentially introduce bias in laboratory confirmed disease cases in some studies. An assay must first be selected by immunologists and validated according to immunological criteria – sensitivity, specificity, reliability, and freedom from intertechnician variability. It may be of interest to know whether the specific immune response measured by the assay is responsible for protection; statistical methods for causal inference have recently been developed allowing an assay to be selected which has been shown to be causally associated with protection [41, 42]. Other considerations include: host factors in which the immune system changes throughout life implying different immune response by age, temporal immunological factors such as timing of measurement and kinetics of the immune response, and population factors given that observed thresholds may not be universally applicable to all settings. Thus, once a correlate of protection or threshold is proposed, further discussions with stakeholders are necessary to cover these diseasespecific considerations that the statistical methods alone cannot address.
A final practical requirement is that datasets to identify immunological correlates of protection are essential. Vaccine efficacy trials provide a clear opportunity to collect data on the relationship between assay values for candidate correlates of protection and disease occurrence; however, they are often sized inadequately to yield convincing conclusions on correlates of protection. Typically trials are designed to capture 40–100 cases of disease to convincingly demonstrate adequate vaccine efficacy against placebo [43–45], but such trials are generally underpowered for assessing correlates of protection. Incorporation of a correlate of protection objective in clinical trials can incur substantial expense to the trial as it would require additional bleeds in subjects after they receive vaccine or placebo to observe their assay values and before any significant number of disease cases occur. Furthermore, more refined titer measures (i.e. less discrete data) would require more serial dilutions and greater blood volumes.
Conclusions
The a:b model together with the evaluation criteria proposed provide a muchneeded set of methods for the estimation and assessment of thresholds values of immunological correlates of protection.
Notes
Declarations
Acknowledgements
The authors wish to thank Dr. Lennart Gustafsson for making available the Swedish household pertussis dataset, Dr. Pedro Piedra for making available the RSV dataset, Dr. James Cherry and Herr Prof. Dr. Ulrich Heininger for permission to use the German pertussis dataset, and Dr. C. Jo White for assistance with the varicella dataset. Dr. Hexin Zhang and Dr. Liou Xu provided suggestions which were incorporated into the work, for which we are grateful. The authors also with to thank the two reviewers, whose comments resulted in valuable improvements to the manuscript.
Authors’ Affiliations
References
 Siegrist CA, Plotkin SA, Orenstein W: The immunology of vaccintion. Vaccines. 2008, Offit PA: Elsevier, 1736. 5Google Scholar
 Advisory Committee for Immunization Practices (ACIP): ACIP Recommendations. http://www.cdc.gov/vaccines/pubs/ACIPlist.htm,
 World Health Organization, Department of Immunization, Vaccines and Biologicals: Immunological basis for immunization series. http://www.who.int/immunization/documents/immunological_basis_series/en]
 Plotkin SA: Correlates of protection induced by vaccination. Clin Vaccine Immunol. 2010, 17 (7): 10551065. 10.1128/CVI.0013110.View ArticlePubMedPubMed CentralGoogle Scholar
 Baylor N: Regulatory perspective: issues of correlates versus surrogates and implications for licensure. 2009, Bamako, Mali: WHO Global Vaccine Research Forum conference workshop on correlates of protection relevant to the African context, http://www.who.int/vaccine_research/documents/WCP_Baylor_presentation.pdf,Google Scholar
 Grand Challenges in Global Health. http://www.grandchallenges.org/NewVaccines/Challenges/LearnaboutImmunologicalResponses/Pages/default.aspx,
 Qin L, Gilbert PB, Corey L, McElrath MJ, Self SG: A framework for assessing immunological correlates of protection in vaccine trials. J Infect Dis. 2007, 196 (9): 13041312. 10.1086/522428.View ArticlePubMedGoogle Scholar
 Gilbert PB, Qin L, Self SG: Evaluating a surrogate endpoint at three levels, with application to vaccine development. Stat Med. 2007, 27: 47584778.View ArticleGoogle Scholar
 Plotkin SA, Gilbert PG: Nomenclature for immune correlates of protection after vaccination. Clin Infect Dis. 2012, 54 (11): 16151617. 10.1093/cid/cis238.View ArticlePubMedPubMed CentralGoogle Scholar
 Dunning A: Experimental designs and statistical methods for postlicensure immunological correlates of protection. 2011, Washington DC: ASA Biopharmaceutical Section FDAIndustry Statistics Workshop, http://www.amstat.org/meetings/fdaworkshop/index.cfm?fuseaction=AbstractDetails&AbstractID=301569,Google Scholar
 Chan I: Assessing the predictive value of immunological markers in vaccines. 2011, Washington DC: ASA Biopharmaceutical Section FDAIndustry Statistics Workshop, http://www.amstat.org/meetings/fdaworkshop/index.cfm?fuseaction=AbstractDetails&AbstractID=301590,Google Scholar
 Björkholm B, Böttiger M, Christenson B, Hagberg L: Antitoxin antibody levels and the outcome of illness during an outbreak of diphtheria among alcoholics. Scand J Infect Dis. 1986, 18 (3): 235239. 10.3109/00365548609032332.View ArticlePubMedGoogle Scholar
 World Health Organization, Department of Immunization, Vaccines and Biologicals: Immunological basis for immunization series. Module 2: Diptheria Update. 2009, http://whqlibdoc.who.int/publications/2009/9789241597869_eng.pdf,Google Scholar
 Chen RT, Markowitz LE, Albrecht P, Stewart JA, Mofenson LM, Preblud SR, Orenstein WA: Measles antibody: reevaluation of protective titers. J Infect Dis. 1990, 162 (5): 10361042. 10.1093/infdis/162.5.1036.View ArticlePubMedGoogle Scholar
 Neumann PW, Weber JM, Jessamine AG, O’Shaughnessy MV: Comparison of measles antihemolysin test, enzymelinked immunosorbent assay, and hemagglutination inhibition test with neutralization test for determination of immune status. J Clin Microbiol. 1985, 22 (2): 296298.PubMedPubMed CentralGoogle Scholar
 Goldschneider I, Gotschlich EC, Artenstein MS: Human immunity to the meningococcus. II. Development of natural immunity. J Exp Med. 1969, 129 (6): 13271348.PubMedGoogle Scholar
 White CJ, Kuter BJ, Ngai A, Hildebrand CS, Isganitis KL, Patterson CM, Capra A, Miller WJ, Krah DL, Provost PJ, Ellis RW, Calandra GB: Modified cases of chickenpox after varicella vaccination: correlation of protection with antibody response. Pediatr Infect Dis J. 1992, 11: 1923. 10.1097/0000645419920100000006.View ArticlePubMedGoogle Scholar
 Siber GR: Methods for estimating serological correlates of protection. Dev Biol Stand. 1997, 89: 283296.PubMedGoogle Scholar
 Jódar L, Butler J, Carlone G, Dagan R, Goldblatt D, Käyhty H, Klugman K, Plikaytis B, Siber G, Kohberger R, Chang I, Cherian T: Serological criteria for evaluation and licensure of new pneumococcal conjugate vaccine formulations for use in infants. Vaccine. 2003, 21 (23): 32653272. 10.1016/S0264410X(03)002305.View ArticlePubMedGoogle Scholar
 Siber GR, Chang I, Baker S, Fernsten P, O’Brien KL, Santosham M, Klugman KP, Madhi SA, Paradiso P, Kohberger R: Estimating the protective concentration of antipneumococcal capsular polysaccharide antibodies. Vaccine. 2007, 25: 38163826. 10.1016/j.vaccine.2007.01.119.View ArticlePubMedGoogle Scholar
 Advisory Committee on Immunization Practices: Licensure of a 13valent pneumococcal conjugate vaccine (PCV13) and recommendations for use among children. MMWR Morb Mortal Wkly Rep. 2010, 59 (9): 258261.Google Scholar
 Andrews N, Borrow R, Miller E: Validation of serological correlate of protection for meningococcal C conjugate vaccine by using efficacy estimates from postlicensure surveillance in England. Clin Diagn Lab Immunol. 2003, 10 (5): 780786.PubMedPubMed CentralGoogle Scholar
 Cherry JD, Gornbein J, Heininger U, Stehr K: A search for serologic correlates of immunity to Bordetella pertussis cough illnesses. Vaccine. 1998, 16 (20): 19011906. 10.1016/S0264410X(98)002266.View ArticlePubMedGoogle Scholar
 Dagan R, GivonLavi N, Fraser D, Lipsitch M, Siber GR, Kohberger R: Serum serotypespecific pneumococcal anticapsular immunoglobulin g concentrations after immunization with a 9valent conjugate pneumococcal vaccine correlate with nasopharyngeal acquisition of pneumococcus. J Infect Dis. 2005, 192 (3): 367376. 10.1086/431679.View ArticlePubMedGoogle Scholar
 Gustafsson L, Hallander HO, Olin P, Reizenstein E, Storsaeter J: A controlled trial of a twocomponent acellular, a fivecomponent acellular, and a wholecell pertussis vaccine. N Engl J Med. 1996, 334 (6): 349355. 10.1056/NEJM199602083340602.View ArticlePubMedGoogle Scholar
 Piedra PA, Jewell AM, Cron SG, Atmar RL, Glezen WP: Correlates of immunity to respiratory syncytial virus (RSV) associatedhospitalization: establishment of minimum protective threshold levels of serum neutralizing antibodies. Vaccine. 2003, 21 (24): 34793482. 10.1016/S0264410X(03)003554.View ArticlePubMedGoogle Scholar
 Stehr K, Cherry JD, Heininger U, SchmittGrohé S, Überall M, Laussucq S, Eckhardt T, Meyer M, Engelhardt R, Christenson P, the Pertussis Vaccine Study Group: A comparative efficacy trial in Germany in infants who received either the Lederle/Takeda acellular pertussis component DTP (DTaP) vaccine, the Lederle wholecell component DTP vaccine, or DT vaccine. Pediatrics. 1998, 101 (1): 111. 10.1542/peds.101.1.1.View ArticlePubMedGoogle Scholar
 Storsaeter J, Hallander HO, Gustafsson L, Olin P: Levels of antipertussis antibodies related to protection after household exposure to Bordetella pertussis. Vaccine. 1998, 16: 19071916. 10.1016/S0264410X(98)002278.View ArticlePubMedGoogle Scholar
 Rapola S, Jantti V, Eerola M, Makela PH, Kayhty H, Kilpi T: AntiPsaA and the risk of pneumococcal AOM and carriage. Vaccine. 2003, 21: 36083613. 10.1016/S0264410X(03)004092.View ArticlePubMedGoogle Scholar
 Jokinen JT, Ahman H, Kilpi TM, Mäkelä PH, Käyhty H: Concentration of antipneumococcal antibodies as a serological correlate of protection: An application to acute otitis media. J Infect Dis. 2004, 190: 545550. 10.1086/422531.View ArticlePubMedGoogle Scholar
 Chan I, Li S, Matthews H, Chan C, Vessey R, Sadoff J, Heyse J: Use of statistical models for evaluating antibody response as a correlate of protection against varicella. Stat Med. 2002, 21 (22): 34113430. 10.1002/sim.1268.View ArticlePubMedGoogle Scholar
 Dunning AJ: A model for immunological correlates of protection. Stat Med. 2006, 25: 14851497. 10.1002/sim.2282.View ArticlePubMedGoogle Scholar
 Coudeville L, Bailleux F, Riche B, Megas F, André P, Ecochard R: Relationship between haemagglutinationinhibiting antibody titres and clinical protection against influenza: development and application of a bayesian randomeffects model. BMC Med Res Methodol. 2010, 10 (1): 1810.1186/147122881018.View ArticlePubMedPubMed CentralGoogle Scholar
 Forrest BD, Pride MW, Dunning AJ, Capeding MR, Chotpitayasunondh T, Tam JS, Rappaport R, Eldridge JH, Gruber WC: Correlation of cellular immune responses with protection against cultureconfirmed influenza virus in young children. Clin Vaccine Immunol. 2008, 15 (7): 10421053. 10.1128/CVI.0039707.View ArticlePubMedPubMed CentralGoogle Scholar
 Black S, Nicolay U, Vesikari T, Knuf M, Del Giudice G, Della Cioppa G, Tsai T, Clemens R, Rappuoli R: Hemagglutination inhibition antibody titers as a correlate of protection for inactivated influenza vaccines in children. Pediatr Infect Dis J. 2011, 30 (12): 10811085. 10.1097/INF.0b013e3182367662.View ArticlePubMedGoogle Scholar
 Miller R, Siegmund D: Maximally selected chi square statistics. Biometrics. 1982, 38: 10111016. 10.2307/2529881.View ArticleGoogle Scholar
 Efron B, Tibshirani R: An Introduction to the Bootstrap. Boca Raton. 1994, : Chapman & Hall/CRC Press LLCGoogle Scholar
 Hosmer DW, Lemeshow S, Klar J: Goodnessoffit testing for the multiple logistic regression when the estimated probabilities are small. Biom J. 1988, 30: 911924. 10.1002/bimj.4710300805.View ArticleGoogle Scholar
 Gershon AA, Takahashi M, Seward J: Varicella Vaccine. Vaccines. Edited by: Plotkin SA, Orenstein W. 2008, Offit PA: Elsevier, 915958. 5Google Scholar
 Goldblatt D: Immunological perspective on correlates for bacterial polysaccharide vaccines: responses, memory and assays. 2009, Bamako, Mali: WHO Global Vaccine Research Forum conference workshop on correlates of protection relevant to the African context, http://www.who.int/vaccine_research/documents/WCP_Goldblatt_presentation.pdf,Google Scholar
 Follmann D: Augmented designs to assess immune response in vaccine trials. Biometrics. 2006, 62 (4): 11611169. 10.1111/j.15410420.2006.00569.x.View ArticlePubMedPubMed CentralGoogle Scholar
 Wolfson J, Peter Gilbert PB: Statistical identifiability and the surrogate endpoint problem. Biometrics. 2010, 66: 11531161. 10.1111/j.15410420.2009.01380.x.View ArticlePubMedPubMed CentralGoogle Scholar
 Black S, Shinefield H, Fireman B, Lewis E, Ray P, Hansen JR, Elvin L, Ensor KM, Hackell J, Siber G, Malinoski F, Madore D, Chang I, Kohberger R, Watson W, Austrian R, Edwards K: Efficacy, safety and immunogenicity of heptavalent pneumococcal conjugate vaccine in children. Pediatr Infect Dis J. 2000, 19 (3): 187195. 10.1097/0000645420000300000003.View ArticlePubMedGoogle Scholar
 Frey S, Vesikari T, SzymczakiewiczMultanowska A, Lattanzi M, Izu A, Groth N, Holmes S: Clinical efficacy of cell culturederived and eggderived inactivated subunit influenza vaccines in healthy adults. Clin Infect Dis. 2010, 51 (9): 9971004. 10.1086/656578.View ArticlePubMedGoogle Scholar
 RuizPalacios GM, PérezSchael I, Velázquez FR: Safety and efficacy of an attenuated vaccine against severe rotavirus gastroenteritis. N Engl J Med. 2006, 354 (1): 1122. 10.1056/NEJMoa052434.View ArticlePubMedGoogle Scholar
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